Human AMSH/STAMBP Alexa Fluor® 488-conjugated Antibody

Catalog #: AF5650G Datasheet / COA / SDS

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AF5650G-100UG

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All AMSH/STAMBP Antibodies

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Human AMSH/STAMBP Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human AMSH/STAMBP in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human AMSH/STAMBP
Ser2-Arg424
Accession # O95630

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AMSH/STAMBP

AMSH (Associated molecule with SH3 domain of STAM) is a 50 kDa member of the peptidase M67C class of enzymes. It is widely expressed and serves two functions. First, it promotes receptor recycling by counteracting the effects of ubiquitinating enzymes, and second, it participates in cytokine signal transduction by forming a complex with Jak2/3, STAM and the common beta - and gamma -chains. Human AMSH is 424 amino acids (aa) in length. It contains an NLS (aa 113‑127), two SH3‑binding motifs (aa 195‑198 and 227‑231), and one MPN domain (aa 252‑361). There are five phosphorylation sites at Ser2, 48, 243, 245 and 247. AMSH has one potential splice variant that shows a 17 aa substitution for aa 1‑68. Full‑length human AMSH is 83% aa identical to mouse AMSH.

Long Name

STAM Binding Protein

Entrez Gene IDs

10617 (Human); 70527 (Mouse); 171565 (Rat)

Alternate Names

AMSH; AMSHAssociated molecule with the SH3 domain of STAM; EC 3.1.2.15; EC 3.4.19.-; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518; STAM binding protein; STAM-binding protein; STAMBP