Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2621E
View all Androgen R/NR3C4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Human Androgen R/NR3C4 peptide

Specificity

Detects human Androgen R/NR3C4 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Androgen R/NR3C4 Antibody

Detection of Human Androgen R/NR3C4 antibody by Western Blot.

Detection of Human Androgen R/NR3C4 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Androgen R/NR3C4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Androgen R/NR3C4 in Human LNCaP Cell Line.

Androgen R/NR3C4 was detected in immersion fixed LNCaP human prostate cancer cell line (left panel; positive staining) and PC‑3 human prostate cancer cell line (right panel; negative staining) using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Androgen R/NR3C4 in Human Prostate Cancer.

Androgen R/NR3C4 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Androgen R/ NR3C4 antibody in LNCaP Human Cell Line antibody by Flow Cytometry.

Detection of Androgen R/ NR3C4 in LNCaP Human Cell Line by Flow Cytometry.

LNCaP human prostate cancer cell line was stained with Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762, filled histogram) or isotype control antibody (MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Detection of Human Androgen R/NR3C4 by Simple WesternTM.

Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Androgen R/NR3C4 at approximately 121 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB58762). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Androgen R/NR3C4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line 

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: LNCaP human prostate cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Simple Western

10 µg/mL
Sample: Lysates of LNCaP human prostate cancer cell line.

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB58762 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Androgen R/NR3C4

The Androgen Receptor (AR) is a 90 kDa steroid hormone receptor that is critical for the development and function of the male reproductive system. AR binding to testosterone or 5 alpha -dihydrotestosterone (DHT) triggers receptor dimerization followed by translocation to the nucleus where it promotes transcription of androgen responsive genes. Multiple polymorphisms in AR are linked to the development of prostate cancer. The ligand binding domain of human AR (aa 661-920) shares 100% aa sequence identity with mouse and rat AR.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human)

Gene Symbol

AR

Additional Androgen R/NR3C4 Products

Product Documents for Human Androgen R/NR3C4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Androgen R/NR3C4 Antibody

For research use only

Customer Reviews for Human Androgen R/NR3C4 Antibody (1)

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  • Human Androgen R/NR3C4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 01/29/2022
    Human Androgen R/NR3C4 Antibody MAB58762

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