Human BAMBI/NMA Antibody

R&D Systems | Catalog # AF921

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BAMBI/NMA Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BAMBI/NMA
Glu27-Ala152
Accession # Q13145

Specificity

Detects human BAMBI/NMA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BAMBI/NMA Antibody

BAMBI/NMA antibody in Human Kidney by Immunohistochemistry (IHC-P).

BAMBI/NMA in Human Kidney.

BAMBI/NMA was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human BAMBI/NMA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF921) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human BAMBI/NMA Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Western Blot

0.1 µg/mL
Sample: Recombinant Human BAMBI/NMA

Reviewed Applications

Read 1 review rated 4 using AF921 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BAMBI/NMA

BAMBI/NMA is a type I transmembrane protein that shares sequence homology with the TGF-beta type I receptor family protein but has a short cytoplasmic region that lacks the kinase domain. It functions as a membrane-bound decoy receptor by competing with type I receptors to form a heterodimer with type II receptors. Mouse BAMBI/NMA share approximately 93% amino acid sequence homology with human BAMBI/NMA.

Long Name

BMP and Activin Membrane-bound Inhibitor

Alternate Names

NMA

Entrez Gene IDs

25805 (Human); 68010 (Mouse)

Gene Symbol

BAMBI

UniProt

Q13145

Additional BAMBI/NMA Products

Product Documents for Human BAMBI/NMA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BAMBI/NMA Antibody

For research use only

Related Research Areas

BMP Family

Citations for Human BAMBI/NMA Antibody

Customer Reviews for Human BAMBI/NMA Antibody (1)

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  • Human BAMBI/NMA Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 08/16/2017
    NMA/BAMBI IF
    Used at 10ug/mL in : 1% BSA/ 0.3% Triton X-100 in PBS overnight at 4C. Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor 555 (used at 1.5ul/mL) 2H, RT in same dilution buffer.
    Human BAMBI/NMA Antibody AF921

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Protocols

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