Human BEND2 Antibody (1121524)

R&D Systems | Catalog # MAB11807

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1121524
View all BEND2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q8NDZ0

Specificity

Detects a synthetic peptide for Human BEND2 around amino acid 550 in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human BEND2 Antibody (1121524)

Immersion fixed paraffin-embedded sections of testis tumor

BEND2 was detected in immersion fixed paraffin-embedded sections of testis tumor using Mouse Anti-Human BEND2 Monoclonal Antibody (Catalog # MAB11807) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human BEND2 Antibody (1121524)

Application
Recommended Usage

Immunohistochemistry

0.25-25 µg/mL
Sample: Immersion fixed paraffin-embbeded sections of human testis tumor

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BEND2

BEN domain-containing protein 2 (BEND2) is a member of the BEND protein family, which is characterized by the presence of BEN domains that play significant roles in chromatin organization and transcriptional regulation. BEND2 is predominantly expressed in the brain and testes, suggesting tissue-specific functions, and has been implicated in gene regulatory processes crucial for cellular identity and development. Dysregulation of BEND2 has been associated with neurodevelopmental disorders, with emerging evidence linking mutations in the BEND2 gene to intellectual disabilities and autism spectrum disorders. Furthermore, its role in chromatin remodeling underscores its importance in maintaining genomic stability and regulating gene expression. Due to these associations, BEND2 may represent a promising biomarker for specific neurological and developmental conditions, and a potential target for therapeutic interventions aimed at modulating chromatin structure and function.

References

1. Ma L, Xie D, Luo M, Lin X, Nie H, Chen J, Gao C, Duo S, Han C. Identification and characterization of BEND2 as a key regulator of meiosis during mouse spermatogenesis. Sci Adv. 2022 May 27;8(21):eabn1606. doi: 10.1126/sciadv.abn1606. Epub 2022 May 25. PMID: 35613276; PMCID: PMC9132480. 
2. Okuno H, Nakashima T, Arai Y, Hama N, Shirakura T, Yamazaki A, Matsumura N, Kirishima M, Fudaba H, Goto H, Hanihara M, Yamasaki K, Yamada S, Washio K, Otani Y, Nagaishi M, Matsuda H, Ishida A, Yamasaki F, Yoshioka T, Yokoo H, Mikami Y, Tanaka S, Hirose T, Sasaki A, Saito R, Arakawa Y, Ishikawa E, Nakada M, Mukasa A, Gomi A, Shibata T, Hirato J, Yoshida A, Suzuki H, Nobusawa S. BEND2 Immunohistochemistry as a Useful Diagnostic Marker for Astroblastomas With BEND2 Fusion. Am J Surg Pathol. 2026 Mar 13. doi: 10.1097/PAS.0000000000002529. Epub ahead of print. PMID: 41821381. 
3. Huang Y, Bucevic N, Coves C, Felipe-Medina N, Marcet-Ortega M, Nikou N, Madrid-Sandín C, López-Panadés M, Buza C, Ferrer Miralles N, Iborra A, Pujol A, Pendás AM, Roig I. The full-length BEND2 protein is dispensable for spermatogenesis but required for setting the ovarian reserve in mice. Elife. 2025 Aug 20;13:RP96052. doi: 10.7554/eLife.96052. PMID: 40833257; PMCID: PMC12367297.

Long Name

BEN domain containing 2

Alternate Names

CXorf20

Entrez Gene IDs

139105 (Human)

Gene Symbol

BEND2

UniProt

Q8NDZ0 (Human)

Additional BEND2 Products

Product Documents for Human BEND2 Antibody (1121524)

Certificate of Analysis

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Product Specific Notices for Human BEND2 Antibody (1121524)

For research use only

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Protocols

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