Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Canine, Hamster

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BMP-2/BMP-4 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP-2
Gln283-Arg396
Accession # P12643

Specificity

Detects BMP-2 and BMP-4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human BMP‑2/BMP‑4 Antibody

Detection of BMP‑2/BMP‑4 in Human Lung Cancer.

BMP‑2/BMP‑4 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human BMP‑2/BMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF355) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human BMP‑2/BMP‑4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed human BMP-2 transfected cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human BMP‑2 (Catalog # 355-BEC)
Recombinant Human BMP‑4 (Catalog # 314-BP)

Reviewed Applications

Read 1 review rated 5 using AF355 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-2/BMP-4

Human BMP-2 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor  beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse and rat BMP-2 are 100% identical. BMP-2 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 114 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. One BMP type II and two BMP type I receptors have been identified. In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both BMP receptor type Is can bind BMP-2 with high-affinity in the absence of BMP receptor type II. 

References

  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49
  2. Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
  3. Reddi, A.H. (1998) Nature Biotechnology 16: 247.

Long Name

Bone Morphogenetic Protein 2/4

Alternate Names

BDA2, BMP-2, BMP2A, BMP-2A, BMP2ABone morphogenetic protein 2A, bone morphogenetic protein 2

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Gene Symbol

BMP2

UniProt

P12643

Additional BMP-2/BMP-4 Products

Product Documents for Human BMP‑2/BMP‑4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human BMP‑2/BMP‑4 Antibody

For research use only

Citations for Human BMP‑2/BMP‑4 Antibody

Customer Reviews for Human BMP‑2/BMP‑4 Antibody (1)

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    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/29/2020
    Human BMP‑2/BMP‑4 Antibody AF355

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Protocols

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