Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BMP-5 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑5

Specificity

Detects human BMP‑5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP-4, rhBMP-6, rhBMP-7, and rhBMP-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human BMP‑5 Antibody

Alkaline Phosphatase Production Induced by BMP‑5 and Neutral-ization by Human BMP‑5 Antibody.

Alkaline Phosphatase Production Induced by BMP‑5 and Neutral-ization by Human BMP‑5 Antibody.

Recombinant Human BMP-5 (Catalog # 615-BMC) induces alkaline phosphatase production in the the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-5 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF615). The ND50 is typically 6-24 µg/mL in the presence of L-ascorbic acid (50 µg/mL).

Applications for Human BMP‑5 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Paraffin-embedded sections of human placenta and osteosarcoma

Western Blot

0.1 µg/mL
Sample: Recombinant Human BMP‑5 (Catalog # 615-BMC)

Neutralization

Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑5 and 50 µg/mL L-ascorbic acid.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-5

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence identity with mouse and rat BMP-5. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (3). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).

References

  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  3. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
  4. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
  5. King, J.A. et al. (1994) Dev. Biol. 166:112.
  6. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
  7. Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
  8. Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
  9. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
  10. Jin, Y. et al. (2001) Oral Oncol. 37:225.
  11. Ro, T.B. et al. (2004) Oncogene 23:3024.

Long Name

Bone Morphogenetic Protein 5

Alternate Names

BMP5

Entrez Gene IDs

653 (Human); 12160 (Mouse)

Gene Symbol

BMP5

Additional BMP-5 Products

Product Documents for Human BMP‑5 Antibody

Certificate of Analysis

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Product Specific Notices for Human BMP‑5 Antibody

For research use only

Related Research Areas

BMP Family

Citations for Human BMP‑5 Antibody

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Protocols

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