Human BMP-7 Antibody (164324) [Biotin]

R&D Systems | Catalog # BAM354

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 164324
View all BMP-7 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7
Arg292-His431
Accession # P18075

Specificity

Detects human BMP-7 in ELISAs. In ELISAs, this antibody shows less than 0.125% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP‑3, rhBMP‑4, rhBMP‑5, rhBMP‑6, rhBMP‑8, rhTGF‑ beta 1, rhTGF‑ beta 2, or rhTGF‑ beta 3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human BMP-7 Antibody (164324) [Biotin]

Human BMP-7 ELISA Standard Curve

Human BMP-7 ELISA Standard Curve

Recombinant Human BMP‑7 (Catalog # 354-BP) was serially diluted and captured by Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BMP‑7 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human BMP-7 Antibody (164324) [Biotin]

Human BMP-7 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human BMP‑7 Antibody (Catalog # MAB3542)
  • Standard: Recombinant Human BMP-7 Protein (Catalog # 354-BP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-7

BMP-7, also known as Osteogenic Protein 1 (OP-1), is one of at least 15 structurally and functionally related BMPs, which are members of the TGF-beta superfamily. BMPs regulate cartilage and bone formation, embryogenesis and morphogenesis of various tissues and organs, and growth, differentiation, chemotaxis, and apoptosis of various cell types. Biologically active BMP-7 is a disulfide-linked homodimer. Cellular responses to BMP-7 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors.

Long Name

Bone Morphogenetic Protein 7

Alternate Names

BMP7, OP-1

Entrez Gene IDs

655 (Human); 12162 (Mouse); 85272 (Rat)

Gene Symbol

BMP7

UniProt

P18075

Additional BMP-7 Products

Product Documents for Human BMP-7 Antibody (164324) [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human BMP-7 Antibody (164324) [Biotin]

For research use only

Citations for Human BMP-7 Antibody (164324) [Biotin]

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