Human Caspase-1 Antibody (661228)

R&D Systems | Catalog # MAB6215

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate - Cercopithecus aethiops (African Green Monkey), Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 661228
View all Caspase-1 Primary Antibodies »

Product Specifications

Immunogen

E.coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466

Specificity

Detects human Caspase-1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Caspase-1 Antibody (661228)

Detection of Human, Mouse, and Rat Caspase-1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Caspase‑1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Caspase-1 antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC).

Caspase‑1 in THP‑1 Human Cell Line.

Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Caspase-1 by Western Blot

Detection of Caspase-1 by Western Blot

NLRP3 was critical to inflammasome activation in response to S. suis P1/7-SLY infection. The THP-1 nlrp3 knockout cell line (THP-1-nlrp3−/−) and its control cell line (THP-1-nlrp3+/+) were primed with LPS and then infected with S. suis strains SC-19, P1/7, P1/7-Vec, P1/7-SLY, P1/7-mSLY or stimulated with ouabain. (A) The cellular proteins and the supernatants of cell cultures were collected for the detection of NLRP3, Casp1, IL-1 beta, and GSDMD via western blot assay. (B) The LDH, IL-1 beta, and TNF-alpha concentrations in the supernatants were also determined (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33792531), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Caspase-1 by Western Blot

Detection of Caspase-1 by Western Blot

Overexpression of SLY but not mSLY in P1/7 causes hyperactivation of inflammasome. THP-1 cells were primed with LPS and then infected with S. suis strains SC-19, msly (P353L), P1/7-Vec, P1/7-SLY, P1/7-mSLY or stimulated with ouabain. (A) The cellular proteins and the supernatants of the cell cultures were collected for the detection of Casp1, IL-1 beta, and GSDMD by western blot assay. (B) The concentrations of LDH, IL-1 beta, and TNF-alpha in the supernatants of THP-1 cells were detected (n = 3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33792531), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Caspase-1 Antibody (661228)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line

Western Blot

0.1 µg/mL
Sample: A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB6215 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caspase-1

Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

Alternate Names

CASP1, Caspase1, ICE

Entrez Gene IDs

834 (Human)

Gene Symbol

CASP1

UniProt

P29466 (Human)

Additional Caspase-1 Products

Product Documents for Human Caspase-1 Antibody (661228)

Certificate of Analysis

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Product Specific Notices for Human Caspase-1 Antibody (661228)

For research use only

Citations for Human Caspase-1 Antibody (661228)

Customer Reviews for Human Caspase-1 Antibody (661228) (1)

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  • Human Caspase-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Spinal motor neurons
    Species: Human
    Verified Customer | Posted 10/06/2021
    Human Caspase-1 Antibody (661228) MAB6215

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