Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all CCL23/MPIF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL23/MPIF-1 (R&D Systems, Catalog # 371-MP)
Arg22-Asn120
Accession # P55773

Specificity

Detects human CCL23/MPIF-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 10% cross-reactivity with recombinant human (rh) CK beta 8-1 (a splice variant of rhMPIF-1) is observed, and less than 0.1% cross-reactivity with rhHCC-1, rhMIP-1 delta, and rhMPIF-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human CCL23/MPIF‑1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP)

Human CCL23/MPIF-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human CCL23/MPIF‑1 Antibody (Catalog # MAB3711)
  • Standard: Recombinant Human CCL23/MPIF-1 Protein (Catalog # 371-MP)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL23/MPIF-1

Myeloid progenitor inhibitory factor (MPIF-1), also known as CK beta 8 and MIP-3, is a member of the CC chemokine subfamily that is designated CCL23. Alternative splicing of the MPIF-1 gene results in two mRNAs that encode a short (CK beta 8) and a long (CK beta 8-1) isoform of the chemokine. CK beta 8 cDNA encodes a 120 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide that is cleaved to generate a 99 aa residue mature CK beta 8 (aa 22 - 120). Additional N-terminal processing of the 99 aa residue variant can generate a 75 aa residue CK beta 8 (aa 46 - 120) that is significantly more active than the 99 aa residue variant. Similarly, CK beta 8-1 encodes a 137 aa residue precursor protein that can give rise to a 116 and a 92 aa residue chemokine. Among CC chemokine members, MPIF-1 is most closely related to MIP-5/CCL15 (67% sequence identity) and MIP-1 alpha /CCL3 (51%). MPIF-1 mRNA is most abundant in the adult lung and liver, but is also present in bone marrow, placenta, and various myelomonocytic cell lines. MPIF-1 has been shown to suppress the low proliferative potential colony-forming cells that give rise to granulocyte and monocyte lineages. MPIF-1 binds to CCR1 with high affinity and has been shown to be a potent chemoattractant and activator of monocytes, dendritic cells, and osteoclast precursors.

References

  1. Patel, V. et al. (1997) J. Exp. Med. 185:1163.
  2. Youn, B-S. et al. (1998) Blood 91:3118.
  3. Nardelli, B. et al. (1999) J. Leukoc. Biol. 61:822.
  4. Berkhout, T.A. et al. (2000) Biochem. Pharmacol. 59:591.

Alternate Names

CCL23, MPIF-1, MPIF1

Entrez Gene IDs

6368 (Human)

Gene Symbol

CCL23

UniProt

P55773

Additional CCL23/MPIF-1 Products

Product Documents for Human CCL23/MPIF‑1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL23/MPIF‑1 Biotinylated Antibody

For research use only

Citations for Human CCL23/MPIF‑1 Biotinylated Antibody

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