Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody

Catalog #: MAB11595 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB11595-100
MAB11595-SP

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Detection of CD20 in HEK293 cells by Flow Cytometry.

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Human CD20 (Research Grade Ocrelizumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Stains hCD20-eGFP transfected HEK293 cells in flow cytometry.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu202

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CD20

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/mL

HEK293 human embryonic kidney cell line transfected with human CD20-eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD20 in HEK293 cells by Flow Cytometry. HEK293 cells transfected with Human CD20-eGFP (A) vs irrelevant-GFP (B) were stained with Human Anti-Human CD20 Monoclonal Antibody (Catalog # MAB11595) followed by Allophycocyanin-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

Ocrelizumab is an anti-human CD20 antibody used for the treatment of advanced or relapsing multiple sclerosis. It is a humanized recombinant anti human CD20 antibody, and is used to specifically deplete B-cells. A proposed pharmacological mechanism of action is that the antibody most likely binds to the B-cell surface CD20 resulting in antibody-dependent cellular cytotoxicity and complement-mediated cell lysis, but does not impact B-cell reconstitution and preexisting humoral immunity.

Long Name

Cluster of Differentiation 20

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Alternate Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7