Human CD31/PECAM-1 Antibody (9G11)

R&D Systems | Catalog # BBA7

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Porcine, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Bioassay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 9G11
View all CD31/PECAM-1 Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human CD31/PEACAM-1. In Western blots, no cross-reactivity with recombinant human (rh) E-Selectin, rhICAM-1, -2, -3, rhVCAM‑1, or recombinant mouse VCAM‑1 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD31/PECAM-1 Antibody (9G11)

Detection of Human CD31/PECAM-1 antibody by Western Blot.

Detection of Human CD31/PECAM‑1 by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for CD31/PECAM-1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD31/PECAM‑1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

CD31/PECAM‑1 in HUVEC Human Cells.

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Mouse Anti-Human CD31/PECAM-1 Monoclonal Antibody (Catalog # BBA7) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CD31/PECAM‑1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line.

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of CD31/PECAM‑1 in Artery in Human Liver.

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of Artery in Human Liver using Mouse Anti-Human CD31/PECAM‑1 Monoclonal Antibody (Catalog # BBA7) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human CD31/PECAM-1 by Immunocytochemistry/ Immunofluorescence

Detection of Human CD31/PECAM-1 by Immunocytochemistry/ Immunofluorescence

Characterization&functional analysis of expanded AEPCs compared with HUVECs. All the experiments performed using AEPCs&HUVECs at passage number 5. (A) Fluorescent immunocytochemistry of the expression profiles of endothelial cell-specific markers, CD31, vWF,&the binding capacity of isolectin-B4, in AEPCs&HUVECs. Nuclear staining was assessed by 4′,6-diamidino-2-phenylindole (DAPI). Photographs taken using a confocal microscope (OLYMPUS, FV1000 with a CCD camera DP71&an objective lens UPlanFL N 40×/1.30 oil; 400× magnification) as a plane image. Bars represent 100 µm. The graphs show the %age of endothelial marker-positive cells among DAPI-positive cells. The data represent two independent experiments (2 donors),&%ages determined from cultivations performed in triplicate. The bars represent means ± SD (n = 6, **P < 0.01, two-tailed unpaired t-test). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35110646), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CD31/PECAM-1 in Human Lung Tissue.

Detection of CD31/PECAM-1 in Human Lung Tissue.

IF staining of CD31/PECAM-1 in human lung tissue. Image from a verified customer review.

Applications for Human CD31/PECAM-1 Antibody (9G11)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Artery in Human Liver

Immunoprecipitation

Lampugnani, M.G. et al. (1992) J. Cell Biol. 118:1511.

Knockout Validated

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line.

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 8 reviews rated 4.3 using BBA7 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD31/PECAM-1

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Entrez Gene IDs

5175 (Human); 18613 (Mouse); 29583 (Rat)

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for Human CD31/PECAM-1 Antibody (9G11)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CD31/PECAM-1 Antibody (9G11)

For research use only

Citations for Human CD31/PECAM-1 Antibody (9G11)

Customer Reviews for Human CD31/PECAM-1 Antibody (9G11) (8)

4.3 out of 5
8 Customer Ratings
5 Stars
38%
4 Stars
50%
3 Stars
13%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 8 reviews Showing All
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  • Pecam1 expression in human lung tissue
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 09/10/2025
    IF staining of pecam 1 in human lung tissue
    IF staining of pecam1 in human lung tissue
    Human CD31/PECAM-1 Antibody (9G11) BBA7
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 03/09/2022
    Human CD31/PECAM-1 Antibody (9G11) BBA7
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human CD31/PECAM-1 Antibody (9G11) BBA7
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 02/28/2017
    Antibody was reconstituted at 0.5mg/ml and used at 1:50 dilution overnight in 4ºC.
    Human CD31/PECAM-1 Antibody (9G11) BBA7
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: artery
    Species: Human
    Verified Customer | Posted 08/29/2016
    Frozen human artery. Permeabilized with 80% acetone. Primary antibody at 1:100 incubate at 4oC overnight Secondary antibody: CY3 donkey anti-mouse secondary antibody. 1 hour at room temperature.
    Human CD31/PECAM-1 Antibody (9G11) BBA7
  • Human CD31/PECAM-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Muscle tissue
    Species: Human
    Verified Customer | Posted 08/29/2016
    Antigen retrieval: 10mM Citrate pH6. Primary antibody at 1:100 incubate at 4oC overnight Secondary antibody: Alexa 647 donkey anti-mouse secondary antibody. 1 hour at room temperature.
    Human CD31/PECAM-1 Antibody (9G11) BBA7

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