Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Chordin-like 2/CHRDL2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chordin-like 2/CHRDL2
Arg28-Thr451
Accession # NP_056239

Specificity

Detects human Chordin-like 2/CHRDL2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Chordin-like 2/CHRDL2 Antibody

Chordin-like 2/CHRDL2 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

Chordin-like 2/CHRDL2 in Human Breast Cancer Tissue.

Chordin-like 2/CHRDL2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human Chordin-like 2/CHRDL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2448) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Chordin-like 2/CHRDL2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Western Blot

0.1 µg/mL
Sample: Recombinant Human Chordin-Like 2/CHRDL2

Reviewed Applications

Read 2 reviews rated 4 using AF2448 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chordin-like 2/CHRDL2

CHRDL2, also known as breast tumor novel factor 1 (BNF-1), is a secreted BMP antagonist implicated in chondrocyte development.

Alternate Names

BNF-1, Chordin like 2, CHRDL2

Entrez Gene IDs

25884 (Human); 69121 (Mouse)

Gene Symbol

CHRDL2

UniProt

NP_056239

Additional Chordin-like 2/CHRDL2 Products

Product Documents for Human Chordin-like 2/CHRDL2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Chordin-like 2/CHRDL2 Antibody

For research use only

Related Research Areas

BMP Family

Customer Reviews for Human Chordin-like 2/CHRDL2 Antibody (2)

4 out of 5
2 Customer Ratings
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4 Stars
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Customer Images

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  • Human Chordin-like 2/CHRDL2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human Chordin-like 2/CHRDL2 Antibody AF2448
  • Human Chordin-like 2/CHRDL2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018

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Protocols

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