Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513)

R&D Systems | Catalog # MAB3651

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 81513
View all CX3CL1/Fractalkine Primary Antibodies »

Product Specifications

Immunogen

NS0 and E. coli-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
Gln25-Arg339 (Ser199Asn)
Accession # P78423

Specificity

Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513)

Detection of CX3CL1/Fractalkine by Flow Cytometry

Detection of CX3CL1/Fractalkine by Flow Cytometry

CX3CL1 expression and regulation in human and murine ovarian cancer cell lines. OV-MZ-6 cells (A, B), OVCAR-3 cells (C), or ID8-Trp53−/− cells (D) were stimulated for 24 h ± TNF-alpha (50 ng/mL) and ± TAPI-2 (50 µM) or ± GI254 (5 µM) or the corresponding solvent controls as indicated. Bar graphs on the left show soluble CX3CL1 in cell supernatant measured after 24 h by ELISA (mean ± SEM). The plots on the right show membrane-bound CX3CL1 as determined via FACS analysis. B, Immunocytochemical staining of CX3CL1 in OV-MZ-6 cells stimulated with or without TNF-alpha in the presence or absence of TAPI-2, visualised by confocal laser scanning microscopy (CLSM) using an Alexa 488-conjugated secondary antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39862711), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CX3CL1/Fractalkine by Flow Cytometry

Detection of CX3CL1/Fractalkine by Flow Cytometry

CX3CL1 expression and regulation in human and murine ovarian cancer cell lines. OV-MZ-6 cells (A, B), OVCAR-3 cells (C), or ID8-Trp53−/− cells (D) were stimulated for 24 h ± TNF-alpha (50 ng/mL) and ± TAPI-2 (50 µM) or ± GI254 (5 µM) or the corresponding solvent controls as indicated. Bar graphs on the left show soluble CX3CL1 in cell supernatant measured after 24 h by ELISA (mean ± SEM). The plots on the right show membrane-bound CX3CL1 as determined via FACS analysis. B, Immunocytochemical staining of CX3CL1 in OV-MZ-6 cells stimulated with or without TNF-alpha in the presence or absence of TAPI-2, visualised by confocal laser scanning microscopy (CLSM) using an Alexa 488-conjugated secondary antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39862711), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of CX3CL1/Fractalkine by Flow Cytometry

Detection of CX3CL1/Fractalkine by Flow Cytometry

CX3CL1 expression and regulation in human and murine ovarian cancer cell lines. OV-MZ-6 cells (A, B), OVCAR-3 cells (C), or ID8-Trp53−/− cells (D) were stimulated for 24 h ± TNF-alpha (50 ng/mL) and ± TAPI-2 (50 µM) or ± GI254 (5 µM) or the corresponding solvent controls as indicated. Bar graphs on the left show soluble CX3CL1 in cell supernatant measured after 24 h by ELISA (mean ± SEM). The plots on the right show membrane-bound CX3CL1 as determined via FACS analysis. B, Immunocytochemical staining of CX3CL1 in OV-MZ-6 cells stimulated with or without TNF-alpha in the presence or absence of TAPI-2, visualised by confocal laser scanning microscopy (CLSM) using an Alexa 488-conjugated secondary antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39862711), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Western Blot

1 µg/mL
Sample: Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # 365-FR)

Reviewed Applications

Read 2 reviews rated 5 using MAB3651 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CX3CL1/Fractalkine

CX3CL1 is a type I membrane protein in which an extracellular chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

UniProt

P78423

Additional CX3CL1/Fractalkine Products

Product Documents for Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513)

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Product Specific Notices for Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513)

For research use only

Citations for Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513)

Customer Reviews for Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513) (2)

5 out of 5
2 Customer Ratings
5 Stars
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Customer Images

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  • Human CX3CL1/Fractalkine Chemokine Domain Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lymphatic endothelium
    Species: Human
    Verified Customer | Posted 08/30/2021
    Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513) MAB3651
  • Name: Gareth Healey
    Application: Immunofluorescence
    Sample Tested: Human HEC1A cells
    Species: Human
    Verified Customer | Posted 01/18/2016
    HEC1A epithelial cells stained with CX3CL1 and an anti-mouse IgG AlexaFluor 488 secondary
    Human CX3CL1/Fractalkine Chemokine Domain Antibody (81513) MAB3651

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