Cytosolic SULT4A1 (Sulfotransferase 4A1 also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes. SULT4A1 message is widely expressed. However, it appears to be translated only in brain. SULT4A is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SULT4A1 is 284 amino acids (aa) in length. A weak PAPS binding site is found between aa 246-254. There are two potential isoforms, one that shows a 13 aa substitution for aa 248-284, and a second that contains a 48 aa substitution for aa 202-247. Full length human SULT4A1 is 98% aa identical to mouse SULT4A1.
Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)
R&D Systems | Catalog # MAB5826
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 634630
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Product Specifications
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 4A1/SULT4A1
Ala2-Lys284
Accession # Q9BR01
Ala2-Lys284
Accession # Q9BR01
Specificity
Detects human Cytosolic Sulfotransferase 4A1/SULT4A1 in direct ELISAs and Western blots. No cross-reactivity with recombinant human (rh) SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, rhSULT2A1, or rhSULT2B1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)
Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot.
Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2 µg/mL of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 4A1/SULT4A1 in Human Brain.
Cytosolic Sulfotransferase 4A1/SULT4A1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody (Catalog # MAB5826) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)
Western Blot
2 µg/mL
Sample: Human brain (cortex) tissue
Sample: Human brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Cytosolic Sulfotransferase 4A1/SULT4A1
Alternate Names
BRSTL1, NST, SULT4A1, SULTX3
Gene Symbol
SULT4A1
UniProt
Additional Cytosolic Sulfotransferase 4A1/SULT4A1 Products
Product Documents for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody (634630)
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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