Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DDX17 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DDX17
Asn498-Tyr638
Accession # Q92841

Specificity

Detects human DDX17 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DDX17 Antibody

Detection of Human DDX17 antibody by Western Blot.

Detection of Human DDX17 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DDX17 at approximately 86 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DDX17 antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

DDX17 in A431 Human Cell Line.

DDX17 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DDX17 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P).

DDX17 in Human Liver Cancer Tissue.

DDX17 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human DDX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4530) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human DDX17 Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver cancer tissue

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DDX17

DDX17 is a member of the DEAD box family of proteins, a family of RNA helicases characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DDX17 plays an essential role in many aspects of cellular RNA metabolism, including RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. Distribution patterns suggests that some members of this family may be involved in spermatogenesis, embryogenesis, and cellular growth and division. Alternative splicing of the DDX17 gene generates two transcript variants encoding isoforms p72 and p82. p82 is translated from a non-AUG start codon 243 bp upstream of the first in frame AUG initiator codon for p72. DDX17 is more closely related to DDX5 than to any other member of the DEAD box family.

Long Name

DEAD Box Protein 17

Alternate Names

p72, RH70

Entrez Gene IDs

10521 (Human); 67040 (Mouse); 315133 (Rat)

Gene Symbol

DDX17

UniProt

Q92841

Additional DDX17 Products

Product Documents for Human DDX17 Antibody

Certificate of Analysis

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Product Specific Notices for Human DDX17 Antibody

For research use only

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Protocols

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