Human Dihydrolipoamide Dehydrogenase/DLD Antibody (1016619)

R&D Systems | Catalog # MAB8646

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1016619
View all Dihydrolipoamide Dehydrogenase/DLD Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human Dihydrolipoamide Dehydrogenase/DLD
Ala36-Phe509
Accession # P09622

Specificity

Detects human Dihydrolipoamide Dehydrogenase/DLD in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Dihydrolipoamide Dehydrogenase/DLD Antibody (1016619)

Dihydrolipoamide Dehydrogenase/DLD antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Dihydrolipoamide Dehydrogenase/DLD in HeLa Human Cell Line.

Dihydrolipoamide Dehydrogenase/DLD was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Dihydrolipoamide Dehydrogenase/DLD Monoclonal Antibody (Catalog # MAB8646) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Dihydrolipoamide Dehydrogenase/DLD antibody in Human Liver Tissue by Immunohistochemistry (IHC-P).

Dihydrolipoamide Dehydrogenase/DLD in Human Liver Tissue.

Dihydrolipoamide Dehydrogenase/DLD was detected in immersion fixed paraffin-embedded sections of human liver tissue using Mouse Anti-Human Dihydrolipoamide Dehydrogenase/DLD Monoclonal Antibody (Catalog # MAB8646) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Dihydrolipoamide Dehydrogenase/DLD Antibody (1016619)

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dihydrolipoamide Dehydrogenase/DLD

Dihydrolipoamide dehydrogenase (DLD), also known as LADH, is an NAD-dependent oxidoreductase in the mitochondrial matrix (1). DLD serves as the E3 subunit of four mitochondrial enzyme complexes: pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, branched chain alpha-ketotacid dehydrogenase, and the glycine cleavage system (2, 3). It is active as a 120 kDa dimer that catalyzes oxidation within these enzyme complexes. Several mutations of human DLD have been described, some of which contribute to the loss of respiratory function during oxidative stress (4, 5). DLD mutations located at the interface between dimer subunits can impair dimer formation (5, 6). The involvement of DLD in the regulation of lipid peroxidation and lactate metabolism is important for mouse hippocampal neuroblast proliferation and hamster sperm capacitation, respectively (7, 8). DLD polymorphisms in insects can increase their resistance to the pesticide phosphine gas, while they can increase the sensitivity to arsenite in the nematode C. elegans (9). Mature human DLD shares 95-96% amino acid (aa) sequence identity with hamster, mouse, and rat DLD. Alternative splicing generates additional human DLD isoforms that lack the N-terminal 99 aa or carry a deletion of aa 147-194.

References

  1. Brand, M.D. (2010) Exp. Gerontol. 45:466.
  2. Johnson, M.T. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14512.
  3. Sundquist, A.R. and R.C. Fahey (1988) J. Bacteriol. 170:3459.
  4. Ambrus, A. et al. (2011) Hum. Mol. Genet. 20:2984.
  5. Vaubel, R.A. et al. (2011) J. Biol. Chem. 286:40232.
  6. Babady, N.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:6158.
  7. Calingasan, N.Y. et al. (2008) Neuroscience 153:986.
  8. Panneerdoss, S. et al. (2012) J. Androl. 33:699.
  9. Schlipalius, D.I. et al. (2012) Science 338:807.

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Entrez Gene IDs

1738 (Human); 13382 (Mouse); 298942 (Rat)

Gene Symbol

DLD

UniProt

P09622

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents for Human Dihydrolipoamide Dehydrogenase/DLD Antibody (1016619)

Certificate of Analysis

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Product Specific Notices for Human Dihydrolipoamide Dehydrogenase/DLD Antibody (1016619)

For research use only

Related Research Areas

Redox Enzymes

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Protocols

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