Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 447506
View all DLL4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DLL4
Ser27-Pro524
Accession # Q9NR61

Specificity

Detects human DLL4 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human DLL4 Antibody

Detection of DLL4 antibody in HUVEC Human Cells antibody by Flow Cytometry.

Detection of DLL4 in HUVEC Human Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells were stained with Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

DLL4 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

DLL4 in HUVEC Human Cells.

DLL4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DLL4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

8-25 µg/mL
Sample:

Immersion fixed HUVEC human umbilical vein endothelial cells

Reviewed Applications

Read 4 reviews rated 4.3 using MAB1506 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL4

Delta-like ligand 4 (DLL4) is a transmembrane protein that contains one DSL domain and eight tandem EGF-like repeats. DLL4 is expressed on arterial endothelial cells where it signals through Notch 1 and Notch 4. DLL4 expression is induced by VEGF and regulates the proliferation of endothelial tip cells during vascular sprouting. DLL4 blockade as well as overexpression can suppress tumor growth by promoting dysregulated angiogenesis. Within the extracellular domain, human DLL4 shares 85% amino acid sequence identity with mouse and rat DLL4.

Long Name

Delta-like 4

Alternate Names

delta 4, Delta 4 precursor, delta ligand 4, delta4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, Drosophila Delta homolog 4, hdelta2, MGC126344, notch ligand delta-2, notch ligand DLL4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Gene Symbol

DLL4

UniProt

Q9NR61

Additional DLL4 Products

Product Documents for Human DLL4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DLL4 Antibody

For research use only

Related Research Areas

Cancer Stem Cell Markers
Notch Pathway

Citations for Human DLL4 Antibody

Customer Reviews for Human DLL4 Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 的 4 reviews Showing All
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  • Human DLL4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E10.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/02/2020
    Staining was done on E10.5 mouse sections (pharyngeal mesoderm). Note the arteries being positive and adjacent veins staining negative for Dll4.
    Human DLL4 Antibody MAB1506
  • Name: Jignesh Ganeshbhai Patel
    Application: Western Blot
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 08/21/2018

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Showing  1 - 4 的 4 reviews Showing All

Protocols

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