Human DOK7 Antibody (651601)

R&D Systems | Catalog # MAB6398

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 651601
View all DOK7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DOK7
Ala179-Pro299
Accession # Q18PE1

Specificity

Detects human DOK7 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human DOK7 Antibody (651601)

DOK7 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

DOK7 in C2C12 Mouse Cell Line.

DOK7 was detected in immersion fixed C2C12 mouse myoblast cell line differentiated into myotubes using Mouse Anti-Human DOK7 Monoclonal Antibody (Catalog # MAB6398) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DOK7 Antibody (651601)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line differentiated into myotubes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DOK7

DOK7 (Downstream of kinase 7) is a 55 kDa member of the DOK family of cytoplasmic adaptor proteins. It links the acetylcholine receptor and the receptor tyrosine kinase MuSK in skeletal and cardiac muscle. Mutations can cause familial myasthenic syndromes. The 504 amino acid (aa) human DOK7 contains pleckstrin homology (aa 7-109) and phosphotyrosine-binding (PTK, aa 125-236) and SH2 domains, and a C-terminal nuclear export signal. Splicing isoforms of 255, 608 and 366 aa diverge at aa 175 or 500, or have 40 divergent aa replacing aa 1-178, respectively. Within aa 179-299, human DOK7 shares 92% and 93% aa identity with mouse and rat DOK7, respectively.

Long Name

Downstream of Tyrosine Kinase 7

Alternate Names

C4orf25, CMS1B

Entrez Gene IDs

285489 (Human); 231134 (Mouse); 305448 (Rat)

Gene Symbol

DOK7

UniProt

Q18PE1 (Human)

Additional DOK7 Products

Product Documents for Human DOK7 Antibody (651601)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DOK7 Antibody (651601)

For research use only

Related Research Areas

Synaptic Proteins and Receptors

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