Human ECE-2 Antibody (233914)

R&D Systems | Catalog # MAB16451

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 233914
View all ECE-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ECE-2
Val104-Leu787
Accession # O60344

Specificity

Detects human ECE-2 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) ECE‑1 and no cross-reactivity with rhKELL is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human ECE-2 Antibody (233914)

ECE‑2 antibody in Human Brain by Immunohistochemistry (IHC-P).

ECE‑2 in Human Brain.

ECE-2 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 3 µg/mL Rat Anti-Human ECE-2 Monoclonal Antibody (Catalog # MAB16451) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human ECE-2 Antibody (233914)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Western Blot

1 µg/mL
Sample: Recombinant Human ECE‑2 (Catalog # 1645-ZN)

Reviewed Applications

Read 1 review rated 5 using MAB16451 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ECE-2

ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.

References

  1. Turner, A.J. et al. (2001) BioEssays 23:261.
  2. Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
  3. Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
  4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Long Name

Endothelin-converting Enzyme-2

Alternate Names

ECE2

Entrez Gene IDs

9718 (Human)

Gene Symbol

ECE2

UniProt

O60344

Additional ECE-2 Products

Product Documents for Human ECE-2 Antibody (233914)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ECE-2 Antibody (233914)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human ECE-2 Antibody (233914)

Customer Reviews for Human ECE-2 Antibody (233914) (1)

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    Sample Tested: See PMID 19541930
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Protocols

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Associated Pathways

A-beta Pathways: Uptake & Degradation
A-beta Pathways: Uptake & Degradation Thumbnail