Human Frk Antibody (393812)

Frk in Human Breast Cancer Tissue.

Frk was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Rat Anti-Human Frk Monoclonal Antibody (Catalog # MAB3766) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in interlobular ducts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Frk by Western Blot

BLK, FGR, HCK, and SRC are expressed in F9 cells. (A) Western blot analysis showing the expression profile of SFKs in F9:iCldn6 cells grown for 96 h in the presence or absence of 1 µg/mL doxycycline (Dox). 293T cells over-expressing the corresponding mouse SFK and Cldn6 genes were used as positive controls (P/C). A breast cancer cell line MDA-MB-231 was used as the positive control for pSFK. (B,C) Confocal immunofluorescent images of the indicated proteins in Dox-exposed F9:iCldin6 cells. Areas consisting of epithelial cells (B) and the boundary regions between undifferentiated and epithelial cells (C) are indicated. Bars, 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37443730), licensed under a CC-BY license. Not internally tested by R&D Systems.