Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2622B
View all GLP-1 Primary Antibodies »

Product Specifications

Immunogen

Human GLP-1 synthetic peptide
Accession # P01275

Specificity

Detects human GLP-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human GLP-1 Antibody

GLP-1 in Human Pancreas Tissue.

GLP-1 was detected in immersion fixed paraffin-embedded sections of human pancreas tissue using Rabbit Anti-Human GLP-1 Monoclonal Antibody (Catalog # MAB10473) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in islets. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Applications for Human GLP-1 Antibody

Application
Recommended Usage

Immunohistochemistry

0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLP-1

Glucagon is a preprotein which is cleaved into four distinct peptides, including the hormone GLP-1 (aa 98-128).  GLP-1 is a secreted hormone with multiple effects upon the intestine (gastric motility), pancreas (glucose dependent insulin release) and hypothalamic pituitary axis (modulates LH, THS, CRH, ocytocin and vasopressin secretion). It also affects plasma glucagon levels.  GLP-1 is expressed in enteroendocrine L cells and neurons of the caudal brainstem which project to the forbain, the amygdala and the hypothalamus. Recent studies show pancreatic intra-islet GLP-1 expression, which is regulated by cytokines, hyperglycemia and cell injury.

Long Name

Glucagon-like Peptide 1

Alternate Names

GLP1

Entrez Gene IDs

2641 (Human)

Gene Symbol

GCG

UniProt

P01275

Additional GLP-1 Products

Product Documents for Human GLP-1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GLP-1 Antibody

For research use only

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Protocols

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