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Human ICAM-3/CD50 Alexa Fluor® 647-conjugated Antibody

Catalog #: BBA15R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA15R-100UG

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Human ICAM-3/CD50 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM‑3/CD50 in ELISAs and Western blots. ICAM‑3/CD50 was found not to recognize COS cells expressing the human
ICAM‑1 protein. The epitope for ICAM‑3/CD50 has been found to be located on domain 1, or the interface between domains 1 a
Source
Monoclonal Mouse IgG1 Clone # ICAM-3.3
Purification
Protein A or G purified
Immunogen
Recombinant human ICAM-3 Fc Chimera
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
 
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
 
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ICAM-3/CD50

Intercellular Adhesion Molecule-3 (ICAM-3, CD50), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and alpha d/ beta 2. ICAM‑3 is expressed on leukocytes and epidermal Langerhans cells. It may play an important role in T cell stimulation by Langerhans cells. ICAM-3 has not been identified in the mouse.

Long Name
Intercellular Adhesion Molecule 3
Entrez Gene IDs
3385 (Human)
Alternate Names
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3

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