Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Binding Assay, Bioassay, Blocking, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 25463
View all IL-4R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R
Gly24-His232
Accession # P24394

Specificity

Detects human IL‑4 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-9 R, recombinant mouse IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL-13 R alpha 1, or rhIL-13 R alpha 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-4R alpha Antibody

Detection of IL-4 Ra antibody in Daudi Human Cell Line antibody by Flow Cytometry.

Detection of IL‑4 R alpha in Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was stained with Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

IL-4 Ra antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

IL‑4 R alpha in Human Lymph Node.

IL-4 Ra was detected in immersion fixed paraffin-embedded sections of human lymph node using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL-4 Ra antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC).

IL‑4 R alpha in HDLM‑2 Human Cell Line.

IL-4 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Mouse Anti-Human IL-4 Ra Monoclonal Antibody (Catalog # MAB230) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 R alpha Antibody.

Recombinant Human IL‑4 (Catalog # 204-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑4 (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑4 Ra Monoclonal Antibody (Catalog # MAB230). The ND50 is typically 3-6 ng/mL.

Detection of Human IL-4R alpha by Flow Cytometry

Detection of Human IL-4R alpha by Flow Cytometry

In vitro binding assays of [V14]6 and [AP1-V12]6 polymer.(A, B) H226, (C, D) MDA-MB-231 and (E, F) H460 cells were incubated with 10 µM of [V14]6, [AP1-V12]6 and AP1 for 1 h at 4°C. Cell binding was determined using flow cytometry. Histograms are representative of three independent experiments. Graphical bars (on right) represent the percent of Alexa 488 labeled polymer bound to cells as mean ±SD of data obtained from three separate experiments performed in triplicates. ***P<0.0001, **P<0.001, and *P<0.05, one-way ANOVA; n = 3. (G, H) H226 cells (3×105 cells) were pre- incubated with different concentrations (1, 5 and 10 µg/mL) of anti-IL-4 receptor antibody followed by 1 h incubation with 10 µM Alexa-labeled [AP1-V12]6 at 4°C. The cells were further suspended in 300 µL of PBS after washing and analyzed using flow cytometry. Histograms are representative of three independent experiments. Graphical bars represent the percent of Alexa 488 labeled polymer bound to cells as mean ±SD of data obtained from three separate experiments performed in triplicates. ***P<0.0001, One way ANOVA; n = 3. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0081891), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-4R alpha by Immunocytochemistry/Immunofluorescence

Detection of Human IL-4R alpha by Immunocytochemistry/Immunofluorescence

In vivo, ex vivo imaging and biodistribution of [AP1-V12]6 polymers.(A) Cy5.5-labeled [V14]6 and [AP1-V12]6 (8 mg/kg) were intravenously injected into MDA-MB-231 tumor xenografted nude mice. Biodistribution was determined by collecting in vivo fluorescence images at different time points. Scale bar indicates normalized fluorescence intensity (NC). Representative optical images of three experiments. (B) Quantitation of fluorescence intensities in tumor sites at respective time points (n = 3). (C) Analysis of fluorescence intensities for tumors and organs from ex vivo images (n = 3). (D) Histological analysis of [AP1-V12]6 polymer (red) localization in tumors. Nuclei were stained with DAPI (blue), and IL-4R expression on cells was visualized by anti-IL-4 receptor antibody staining (green). Representative confocal images of three experiments (scale bar 50 µm). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0081891), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-4R alpha Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HDLM-2 human Hodgkin's lymphoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑4 R alpha (Catalog # 230-4R) under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 3-6 ng/mL in the presence of 0.2 ng/mL Recombinant Human IL‑4.

Reviewed Applications

Read 4 reviews rated 4.5 using MAB230 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-4R alpha

Interleukin 4 receptor (IL-4 R) is the transmembrane ligand binding subunit of the heterodimeric IL-4 receptor complex. In the type I receptor complex, IL-4 R associates with the common gamma chain, whereas in the type II receptor complex, it associates with IL-13 R alpha 1. IL-4 R is expressed by B cells, T cells, monocytes and macrophages.

Long Name

Interleukin 4 Receptor alpha

Alternate Names

CD124, IL-4 R alpha, IL-4Ra, IL4R, IL4R alpha

Entrez Gene IDs

3566 (Human); 16190 (Mouse); 102133649 (Cynomolgus Monkey)

Gene Symbol

IL4R

UniProt

P24394 (Human)

Additional IL-4R alpha Products

Product Documents for Human IL-4R alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-4R alpha Antibody

For research use only

Citations for Human IL-4R alpha Antibody

Customer Reviews for Human IL-4R alpha Antibody (4)

4.5 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human IL-4R alpha Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 的 4 reviews Showing All
Filter By:
Clear Filters X
  • Human IL-4R alpha Antibody
    Name: Anonymous
    Application: CyTOF
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 05/04/2022
    Gated on CD45+ PBMCs
    Human IL-4R alpha Antibody MAB230
  • Human IL-4R alpha Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: LAD2 human mast cells
    Species: Human
    Verified Customer | Posted 04/07/2022
    Human IL-4R alpha Antibody MAB230
  • Human IL-4R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Colon cancer cells
    Species: Human
    Verified Customer | Posted 08/19/2021
    Human IL-4R alpha Antibody MAB230
  • Human IL-4 R alpha Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 12/06/2017
    Human IL-4R alpha Antibody MAB230

There are no reviews that match your criteria.

Showing  1 - 4 的 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies