Human Integrin alpha 4/CD49d Antibody

Catalog #: MAB1354
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1354-SP
MAB1354-100
MAB1354-500

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Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry.

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Citations (7)

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Human Integrin alpha 4/CD49d Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 4/CD49d. Recognizes the human Integrin alpha 4/CD49d subunit.

Source

Monoclonal Mouse IgG₁ Clone # 7.2R

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

DX-3 human melanoma cell line

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 4/CD49d in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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Integrin alpha 4/CD49d in U937 Human Cell Line. Integrin a4/ CD49d was detected in immersion fixed U937 human histiocytic lymphoma cell line using 10 µg/mL Mouse Anti-Human Integrin a4/CD49d Monoclonal Antibody (Catalog # MAB1354) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4/CD49d

The alpha 4 subunit, also known as CD49d and VLA-4 alpha subunit, forms heterodimers with Integrin beta 1 (CD29) or beta 7. Integrins alpha 4 beta 1 and alpha 4 beta 7 are receptors for fibronectin and VCAM. Integrin alpha 4 beta 1 also binds the mucosal addressin cell adhesion molecule (MAdCAM-1).

Entrez Gene IDs

3676 (Human); 16401 (Mouse); 102128433 (Cynomolgus Monkey)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); ITGA4; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha; VLA-4

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Integrin alpha 4/CD49d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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&alpha2&beta1 integrins spatially restrict Cdc42 activity to stabilise adherens junctions
Authors: JD Howden, M Michael, W Hight-Warb, M Parsons
Bmc Biology, 2021-06-23;19(1):130.
The Force-Dependent Mechanism of an Integrin ?4?7-MAdCAM-1 Interaction
Authors: Su, Y;Luo, Z;Sun, D;Yang, B;Li, Q;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
J. Clin. Invest., 2019-05-14;129(7):2745-2759.
Species: Human
Sample Types: Protein
Applications: Bioassay
C6-ceramide nanoliposome suppresses tumor metastasis by eliciting PI3K and PKCzeta tumor-suppressive activities and regulating integrin affinity modulation.
Authors: Zhang P, Fu C, Hu Y, Dong C, Song Y, Song E
Sci Rep, 2015-03-20;5(0):9275.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis Rheum., 2007-06-01;56(6):1817-26.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Authors: van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Dig. Dis. Sci., 2007-03-30;52(10):2775-83.
Species: Human
Sample Types: Whole Cells
Applications: ICC
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Authors: Carman CV, Springer TA
J. Cell Biol., 2004-10-25;167(2):377-88.
Species: Human
Sample Types: Whole Cells
Applications: ICC