Human Integrin alpha V beta 5 Antibody (P5H9)

R&D Systems | Catalog # MAB2528

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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Transgenic Mouse

Applications

Validated:

Adhesion Blockade, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Immunohistochemistry, Neutralization, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P5H9
View all Integrin alpha V beta 5 Primary Antibodies »

Product Specifications

Immunogen

HT1080 human fibrosarcoma cell line

Specificity

Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Integrin alpha V beta 5 Antibody (P5H9)

Integrin aV beta 5 antibody in HT1080 Human Cell Line by Immunocytochemistry (ICC).

Integrin alpha V beta 5 in HT1080 Human Cell Line.

Integrin aV beta 5 was detected in immersion fixed HT1080 human fibrosarcoma cell line using Human Integrin aV beta 5 Monoclonal Antibody (Catalog # MAB2528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Integrin alpha V beta 5 by Immunocytochemistry/Immunofluorescence

Detection of Human Integrin alpha V beta 5 by Immunocytochemistry/Immunofluorescence

alpha V beta 3 and EGFR mediate MBsome signaling. a–d HeLa cells were incubated with purified GFP-MBs for 3 h, followed by wash and another incubation for 24 h. Cells were then fixed and stained with anti-alpha V beta 3 (a), anti-alpha V beta 5 (b), anti-phospho-FAK (d) antibodies. Panels in c show control staining where primary antibodies were not added. Boxed regions mark the part of the image shown as a higher magnification image in the insets on the right. Scale bar is equivalent to 1 μm. e HeLa cells were co-incubated with purified GFP-MBs and EGF-Alexa647, followed by wash and another incubation for 24 h. Cells were then fixed and colocalization between MBs and EGF analyzed. Arrows point to MBsomes. f HeLa cells were co-incubated with purified GFP-MBs and non-labeled EGF, followed by wash and another incubation for 24 h. Cells were then fixed and stained with anti-phospho-EGFR antibodies. Arrows point to MBsomes. Scale bars in insets are equivalent to 2 μm. g HeLa cells were incubated with purified GFP-MBs. Cells were then washed and flow sorted to separate fractions with or without internalized GF-MBs. Equal number of cells from each fraction were then plated and incubated for 48 h in the presence or absence of 10 μm of EGFR inhibitor (erlotinib). Cells were then washed again and incubated for another 48 h followed by cell counting to determine the number of cells. Data shown are the means and standard deviations derived from three independent experiments (one-way ANOVA) Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31320617), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Integrin  alpha V beta 5 in HT1080 cells by Flow Cytometry

HT1080 cells were stained with Mouse Anti-Human Integrin  alpha V beta 5 Monoclonal Antibody (Catalog # MAB2528) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of Integrin alpha V beta 5 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human Integrin aV beta 5 Monoclonal Antibody (Catalog # MAB2528, filled histogram) or isotype control antibody (IC002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Integrin alpha V beta 5 Antibody (P5H9)

Application
Recommended Usage

Adhesion Blockade

Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HT1080 human fibrosarcoma cell line and MCF‑7 human breast cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HT1080 human fibrosarcoma cell line, M21 human melanoma cell line, and H2981 human lung carcinoma cell line

Immunoprecipitation

Wayner, E.A. et al. (1991) J. Cell Biol. 113:919.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB2528 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 5

Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.

Alternate Names

CD51, integrin subunit alpha V, ITGAV, MSK8, VNRA, VTNR

Entrez Gene IDs

3685 (Human)

Gene Symbol

ITGAV

Additional Integrin alpha V beta 5 Products

Product Documents for Human Integrin alpha V beta 5 Antibody (P5H9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin alpha V beta 5 Antibody (P5H9)

For research use only

Citations for Human Integrin alpha V beta 5 Antibody (P5H9)

Customer Reviews for Human Integrin alpha V beta 5 Antibody (P5H9) (2)

4.5 out of 5
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Showing  1 - 2 的 2 reviews Showing All
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  • Human Integrin  alpha V beta 5 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: DU145 human prostate carcinoma cell line and PC-3 human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 11/12/2025
    Human Integrin alpha V beta 5 Antibody (P5H9) MAB2528
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Human Integrin  alpha V beta 5 Antibody
    Name: Adam Guess
    Application: Flow Cytometry
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 01/10/2017
    Human Integrin alpha V beta 5 Antibody (P5H9) MAB2528

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