Human L1CAM Alexa Fluor™ Plus 647‑conjugated Antibody

R&D Systems | Catalog # FAB7773AFP647

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2702C
View all L1CAM Primary Antibodies »

Product Specifications

Specificity

Detects human L1CAM in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for Human L1CAM Alexa Fluor™ Plus 647‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Flow Cytometry

Background: L1CAM

L1CAM (Neural cell adhesion molecule L1, also known as L1, CD171 and NCAM-L1) is a founding member of the L1 family, Immunoglobulin (Ig) superfamily of molecules (1-4). It was initially described as a 200-230 kDa neural adhesion molecule that likely played a key role in mouse nervous system development (4-6). Subsequent studies have confirmed the adhesive nature of the molecule, and expanded its activities in both neural and nonneural cell types. L1 is now recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation (1, 7, 8). It does so through a series of cis and trans interactions that involve multiple copartners and target receptors (1, 3, 6, 8). Cells known to express L1 are varied, and include immature oligodendrocytes (9), CD4+ T cells, B cells and monocytes (10), both motor and sensory Schwann cells (11, 12), intestinal epithelial progenitor cells (12), cerebellar granule and Purkinje cells (5, 13, 14), and multiple tumor cells such as melanoma (15) plus pancreatic duct and lung carcinoma cells (16, 17). Human L1 was first identified as a 215 kDa glycoprotein on the surface of SKNAS neuroblastoma cells (18). Subsequent cloning established its precursor as being 1257 amino acids (aa) in length (19, 20). The molecule is a type I transmembrane (TM) protein that contains an 1101 aa extracellular region (aa 20-1120) plus a 114 aa cytoplasmic domain (aa 1144-1257). The extracellular region possesses six C2-type Ig-like domains (aa 35-607) followed by five fibronectin (FN) type III domains (aa 612-1108). As noted, L1 participates in multiple cis and trans interactions, and some of these interaction sites have been mapped to select Ig or FN domains. For instance, Ig-like domains #1, 2 and 6 associate with NP-1, L1 (homotypic binding), and various integrins, respectively (21‑23). The latter interaction is mediated by one (in human) or two (in mouse) RGD motifs (23, 24). Other molecules that heterotypically associate with L1 include NCAM, neurocan, CD24 and EGFR. The cytoplasmic tail contains no kinase motifs, but does possess a FIGQY peptide that interacts with ankyrin, and an RSLE sequence that mediates clathrin-associated endocytosis (1). There are two splice variants, one each in the intracellular and extracellular domains.  A deletion of RSLE adversely affects endocytosis, while a Leu substitution for aa 26-31 interfers with numerous heterotypic interactions (25, 26).  In general, the full-length L1 molecule is a neuron-associated isoform. L1 is known to undergo proteolysis, either by plasmin or ADAMs. This generates soluble isoforms of varying sizes (140-200 kDa) that retain bioactivity, and which can be incorporated into the surrounding ECM (5, 13, 27-30). The membrane fragments (30-80 kDa) undergo further processing, most importantly by gamma -secretase, to generate a soluble 28 kDa intracellular domain. This domain is SUMOylated, and believed to possess an NLS at Lys1147.  Upon presumed entry into the nucleus, L1 is posited to activate L1-responsive genes. Human and mouse L1 precursors share 88% aa sequence identity.

References

  1. Maness, P.F. and M. Schachner (2007) Nat. Neurosci. 10:19.
  2. Wei, C.H. and S.E. Ryu (2012) Exp. Mol. Med. 44:413.
  3. Faspel, J. and M. Grumet (2003) Front. Biosci. 8:1210.
  4. Herron, L.R. et al. (2009) Biochem. J. 419:519.
  5. Rathjen, F.G. and M. Schachner (1984) EMBO. J. 3:1.
  6. Keifel, H. et al. (2011) Trends Mol. Med. 17:178.
  7. Dihne, M. et al. (2003) J. Neurosci. 23:6638.
  8. Kadmon, G. et al. (1998) Dev. Immunol. 6:205.
  9. Itoh, K. et al. (2000) J. Neurosci. Res. 60:579.
  10. Jouet, M. et al. (1995) Mol. Brain Res. 30:378.
  11. He, Q. et al. (2012) Neurosci. Lett. 521:57.
  12. Thor, G. et al. (1987) EMBO J. 6:2581.
  13. Sadoul, K. et al. (1988) J. Neurochem. 50:510.
  14. Hubbe, M. et al. (1993) Eur. J. Immunol. 23:2927.
  15. Hoja-Lukowicz, D. et al. (2012) Glycoconj J. Apr 29. [Epub ahead of print].
  16. Geismann, C. et al. (2009) Cancer Res. 69:4517.
  17. Tischler, V. et al. (2011) Mol. Cancer 10:127.
  18. Mujoo, K. et al. (1986) J. Biol. Chem. 261:10299.
  19. Kobayashi, M. et al. (1991) Biochim. Biophys. Acta 1090:238.
  20. Hlavin, M.L. and V. Lemmon (1991) Genomics 11:416.
  21. Castellani, V. et al. (2002) EMBO J. 21:6348.
  22. Zhao, X. and C-H Siu (1995) J. Biol. Chem. 270:29413.
  23. Oleszewski, M. et al. (1999) J. Biol. Chem. 274:24602.
  24. Felding-Habermann, B. et al. (1997) J. Cell Biol. 139:1567.
  25. Kamiguchi, H. et al. (1998) J. Neurosci. 18:5311.
  26. De Angelis, E. et al. (2001) J. Biol. Chem. 276:32738.
  27. Montgomery, A.M.P. et al. (1996) J. Cell Biol. 132:475.
  28. Sadoul, R. et al. (1989) J. Neurochem. 53:1471.
  29. Reidle, S. et al. (2009) Biochem. J. 420:391.
  30. Lutz, D. et al. (2012) J. Biol. Chem. 287:17161.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Entrez Gene IDs

3897 (Human); 16728 (Mouse); 50687 (Rat)

Gene Symbol

L1CAM

UniProt

CAA42508

Additional L1CAM Products

Product Documents for Human L1CAM Alexa Fluor™ Plus 647‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human L1CAM Alexa Fluor™ Plus 647‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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