Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2)

R&D Systems | Catalog # MAB2144

R&D Systems
Discontinued Product
MAB2144 has been discontinued. View all Laminin alpha 3/Laminin-5 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Adhesion Blockade, Immunocytochemistry, Immunoprecipitation

Cited:

Immunohistochemistry, Neutralization, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P3H9-2
View all Laminin alpha 3/Laminin-5 Primary Antibodies »

Product Specifications

Immunogen

Human keratinocytes

Specificity

Detects human Laminin alpha 3/Laminin‑5 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2)

Laminin a3/Laminin-5 antibody in MCF 10A Human Cell Line by Immunocytochemistry (ICC).

Laminin alpha 3/Laminin-5 in MCF 10A Human Cell Line.

Laminin a3/Laminin-5 was detected in immersion fixed MCF 10A human breast epithelial cell line using 10 µg/mL Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Laminin a3/Laminin-5 antibody in Human Skin by Immunohistochemistry (IHC-P).

Laminin alpha 3/Laminin‑5 in Human Skin.

Laminin a3/Laminin-5 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Laminin a3/Laminin-5 Monoclonal Antibody (Catalog # MAB2144) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to cell in the epiderimis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2)

Application
Recommended Usage

Adhesion Blockade

Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF 10A human breast epithelial cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Immunoprecipitation

Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. 86:4654.

Western Blot

Wayner, E.A. (1993) J. Cell Biol. 121(5):1141; Gallatin, W.M. et al. (1989) Proc. Natl. Acad. Sci. USA 86(12):4654. This application was not tested by R&D Systems.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB2144 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Laminin alpha 3/Laminin-5

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with Integrins, Dystroglycan and other receptors, Laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival (1). Laminin-5, also known as Epiligrin, consists of an alpha 3, beta 3, and gamma 2 chain. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and plays an important role in the initiation and maintenance of epithelial cell anchorage to the underlying connective tissue (2, 3).

References

  1. Colognato, H. et al. (2000) Dev. Dyn. 218:213.
  2. Stahl, S. et al. (1997) J. Cell Sci. 110:55.
  3. Gonzales, M. et al. (1999) Mol. Biol. Cell 10:259.

Alternate Names

Epiligrin, LAMA3, Laminin-5, Laminin5

Entrez Gene IDs

3909 (Human)

Gene Symbol

LAMA3

Additional Laminin alpha 3/Laminin-5 Products

Product Documents for Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2)

For research use only

Related Research Areas

Extracellular Matrix Molecules

Citations for Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2)

Customer Reviews for Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
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2 Stars
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1 Stars
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Showing  1 - 3 的 3 reviews Showing All
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  • Human Laminin  alpha 3/Laminin-5 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Colonic cancer cells
    Species: Human
    Verified Customer | Posted 07/22/2021
    Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2) MAB2144
  • Human Laminin  alpha 3/Laminin-5 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Embryonic basement membrane
    Species: Human
    Verified Customer | Posted 07/08/2021
    Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2) MAB2144
  • Human Laminin  alpha 3/Laminin-5 Antibody
    Name: Michelle Chen
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HUVEC human umbilical vein endothelial cells
    Species: Human
    Verified Customer | Posted 04/26/2017
    Human Laminin alpha 3/Laminin-5 Antibody (P3H9-2) MAB2144

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Protocols

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