Human LINGO-2 (LRR and Ig domain-containing, Nogo Receptor-interacting protein 2; also known as Leucine-rich repeat neuronal 6C (LRRN6C) or LERN3), type I transmembrane protein in the neuronal leucine-rich repeat family. These proteins have a signal peptide, 12 extracellular leucine-rich repeats flanked by N-terminal and C-terminal cysteine-rich sequences, an immunoglobulin-like domain, a transmembrane domain and a short cytoplasmic tail. An alternate start site may exist at Met148 of the precursor. Human LINGO-2 is a highly conserved, 606 amino acid protein that shares 99% and 98% aa sequence identity with canine and mouse LINGO-2, respectively. LINGO-2 presumably functions outside the CNS with little involvement by p75/NgR1.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 382007
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LINGO-2
Cys28-Leu542
Accession # Q7L985
Cys28-Leu542
Accession # Q7L985
Specificity
Detects human LINGO-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human LINGO-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human LINGO‑2 Antibody
Detection of Human LINGO‑2 by Western Blot.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LINGO-2 Monoclonal Antibody (Catalog # MAB36791) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LINGO-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of LINGO‑2 in HEK293 Human Cell Line Transfected with Human LINGO-2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line either (A) transfected with human LINGO-2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human LINGO-2 Monoclonal Antibody (Catalog # MAB36791) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003).
Applications for Human LINGO‑2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human LINGO-2 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human LINGO-2 and eGFP
Western Blot
2 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line
Sample: SH‑SY5Y human neuroblastoma cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: LINGO-2
Long Name
Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 2
Alternate Names
LERN3, LINGO2, LRRN6C
Gene Symbol
LINGO2
UniProt
Additional LINGO-2 Products
Product Documents for Human LINGO‑2 Antibody
Product Specific Notices for Human LINGO‑2 Antibody
For research use only
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