Human LRRK2 Antibody

LRRK2 (Leu-rich repeat/LRR-containing protein kinase 2; also Dardarin) is a 260-280 kDa member of the TKL (Tyr kinase-like) Ser/Thr protein kinase family of molecules. It is unusual in that it possesses both GTPase and protein kinase enzyme activity. LRRK2 is expressed in dopamine system neurons, macrophages and B2 B cells (in rodent), and mutations of the molecule are associated with the pathogenesis of Parkinson's disease. Through its protein kinase activity, LRRK2 phosphorylates both 4E-BP and FoxO1, two molecules involved in the initiation of protein translation. Human LRRK2 is 2527 amino acids (aa) in length. It contains multiple domains, including 16 LRRs (aa 226-249; 791-1291; 1556-1887), one coiled-coil region (aa 319-348), a GTPase Roc domain (aa 1328-1511), a protein kinase region (aa 1879-2138), and a WD40 domain (aa 2168-2387). There are three potential splice variants for LRRK2. One shows an alternative start site at Met1203 coupled to a 26 aa substitution for aa 2465-2527, a second contains a three aa substitution for aa 1-1165, and a third possesses an alternative start site at Met402 accompanied by a 12 aa substitution for aa 1259-2527. Over aa 241-500, human LRRK2 shares 85% aa identity with mouse LRRK2.