Human MKK7 Antibody

Catalog # Availability Size / Price Qty
MKK7 in Human Tonsil.
1 Image
Product Details
Supplemental Products

Human MKK7 Antibody Summary

Species Reactivity
Detects rhMKK7 in direct ELISAs. This antibody does not cross-react with rhMKK4.
Monoclonal Mouse IgG2B Clone # 324302
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human MKK7
Accession # O14733
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MKK7 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

MKK7 in Human Tonsil. MKK7 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human MKK7 Monoclonal Antibody (Catalog # MAB3579) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK7

Mitogen-activated protein kinase kinase 7 (MKK7 or MAP2K7), also known as Jun kinase kinase 2 (JNKK2), is activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation at Ser 271 and Thr 275 by several upstream MAPK kinase kinases (MAP3Ks). MKK7 is a dual specificity protein kinase, phosphorylating and activating the JNK family of MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Pro-Tyr.

Long Name
Mitogen-activated Protein Kinase Kinase 7
Entrez Gene IDs
5609 (Human); 26400 (Mouse); 363855 (Rat)
Alternate Names
c-Jun N-terminal kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 7; EC; JNK kinase 2; JNK-activating kinase 2; JNKK 2; JNKK2; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK7; MEK 7; MEK7; mitogen-activated protein kinase kinase 7; MKE7; MKK7; MKK7MAPKK 7; PRKMK7

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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