Human MKP-1 Antibody

Catalog #: MAB7168 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7168
MAB7168-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MKP-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human MKP-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) MKP-2, rhMKP-3, or rhMKP-5 is observed.

Source

Monoclonal Rat IgG_2A Clone # 408612

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human MKP-1
Met1-Cys367
Accession # P28562

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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MKP‑1 in Human Prostate. MKP-1 was detected in immersion fixed paraffin-embedded sections of human prostate using Rat Anti-Human MKP-1 Monoclonal Antibody (Catalog # MAB7168) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKP-1

MKP-1, also known as PTPN10, DUSP1, and CL100, is an approximately 45 kDa Ser/Thr protein phosphatase that dephosphorylates p38 MAPK and JNK. Acetylation of MKP-1 enhances this activity and limits the inflammation-induced signaling downstream of TLR proteins. Within aa 1-367, human MKP-1 shares 97% aa sequence identity with mouse and rat MKP-1.

Long Name

MAP Kinase Phosphatase 1

Entrez Gene IDs

1843 (Human); 19252 (Mouse); 114856 (Rat)

Alternate Names

CL100; CL100MAP kinase phosphatase 1; dual specificity phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP1; EC 3.1.3.16; EC 3.1.3.48; HVH1; Mitogen-activated protein kinase phosphatase 1; MKP1; MKP-1; MKP-1dual specificity protein phosphatase 1; Protein-tyrosine phosphatase CL100; PTPN10; PTPN10serine/threonine specific protein phosphatase; VH1