Human MMP-10 Antibody (110304)

R&D Systems | Catalog # MAB910

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 110304
View all MMP-10 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP-10
Tyr18-Cys476
Accession # P09238

Specificity

Detects human MMP-10 in Western blots. Does not cross-react with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MMP-10 Antibody (110304)

MMP‑10 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑10 in Human Colon Cancer Tissue.

MMP‑10 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Human MMP‑10 Monoclonal Antibody (Catalog # MAB910) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MMP-10 Antibody (110304)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Recombinant Human MMP‑10 Western Blot Standard (Catalog # WBC026)

Reviewed Applications

Read 2 reviews rated 3 using MAB910 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-10

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain.

Long Name

Matrix Metalloproteinase 10

Alternate Names

MMP10, Stromelysin 2

Entrez Gene IDs

4319 (Human)

Gene Symbol

MMP10

UniProt

P09238

Additional MMP-10 Products

Product Documents for Human MMP-10 Antibody (110304)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-10 Antibody (110304)

For research use only

Citations for Human MMP-10 Antibody (110304)

Customer Reviews for Human MMP-10 Antibody (110304) (2)

3 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
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2 Stars
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1 Stars
50%

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Customer Images

Showing  1 - 2 的 2 reviews Showing All
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  • Human MMP-10 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon cancer tissue
    Species: Human
    Verified Customer | Posted 02/13/2022
    Human MMP-10 Antibody (110304) MAB910
  • Human MMP-10 Antibody
    Name: avinash waghray
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: human lung normal
    Species: Human
    Verified Customer | Posted 04/25/2018
    I checked it on human lung sample and it did not work. I used it with no retrieval, citrate retrieval and EDTA retrieval. All did not work.
    Human MMP-10 Antibody (110304) MAB910
    Bio-Techne Response
    Technical Service will be following up with this customer.

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Protocols

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