Human MMP-11 Antibody (135421)

R&D Systems | Catalog # MAB3657

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 135421
View all MMP-11 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MMP‑11
Met1-Leu488
Accession # P24347

Specificity

Detects human MMP-11 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human MMP-11 Antibody (135421)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 4 reviews rated 4.3 using MAB3657 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-11

MMP-11, also called Stromelysin-3, is a metalloproteinase secreted by cells of mesenchymal origin. Human MMP-11 contains a 31 amino acid (aa) signal sequence, a 65 aa propeptide cleaved at a furin consensus sequence, and a 390 aa mature sequence with zinc metalloproteinase and hemopexin-like domains. The human 45 kDa mature MMP-11 shows 89% aa identity with mouse. An inducible low-abundance 40 kDa isoform is a constitutively active intracellular form. MMP-11 is upregulated in stromal cells near many invasive carcinomas, where it can cleave IGFBP, allowing IGF-1 to promote tumor cell growth.

Long Name

Matrix Metalloproteinase 11

Alternate Names

MMP11

Entrez Gene IDs

4320 (Human)

Gene Symbol

MMP11

UniProt

P24347

Additional MMP-11 Products

Product Documents for Human MMP-11 Antibody (135421)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-11 Antibody (135421)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human MMP-11 Antibody (135421)

Customer Reviews for Human MMP-11 Antibody (135421) (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 的 4 reviews Showing All
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  • Human MMP-11 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human MMP-11 Antibody (135421) MAB3657
  • Human MMP-11 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human MMP-11 Antibody (135421) MAB3657
  • Human MMP-11 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/14/2018
  • Human MMP-11 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018

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