Human MMP-16/MT3-MMP Antibody

R&D Systems | Catalog # AF1785

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-16/MT3-MMP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MMP‑16/MT3‑MMP
Ala32-Gly291 (Ile152Asn)
Accession # P51512

Specificity

Detects human MMP‑16/MT3‑MMP in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP-16/MT3-MMP Antibody

MMP-16/MT3-MMP antibody in Human Breast by Immunohistochemistry (IHC-P).

MMP‑16/MT3‑MMP in Human Breast.

MMP-16/MT3-MMP was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human MMP-16/MT3-MMP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1785) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of MMP-16/MT3-MMP by Western Blot

Detection of MMP-16/MT3-MMP by Western Blot

Mechanism: effect of miR-193b-3p’s target gene MMP16 on chondrocyte sheets. (A) Sequence of the predicted miR-193b-3p binding site with the 3’UTR of MMP16 (position 2232-2238). (B) Vectors used in dual-luciferase reporter assay containing the seed sequence of MMP16 3’UTR. (C) Reduced relative luciferase activity transfected with MMP16 and miR-193b-3p, compared with the negative control or MMP16 mutant (p < 0.05) (n=3). (D, E) Gene expression of COL2A1 and MMP16 (p < 0.05) (n=3). (F) Protein expression of COL2A1 and MMP16 for chondrocytes cultured in different groups. (G, H) Quantitative protein level of COL2A1 and MMP16 in different groups (p < 0.05) (n=3). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31492826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP-16/MT3-MMP Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑16/MT3-MMP (Catalog # 1785-MP), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human MMP‑16/MT3-MMP (Catalog # 1785-MP)

Reviewed Applications

Read 1 review rated 5 using AF1785 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-16/MT3-MMP

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix (ECM). MMP-16 (MT3-MMP) is found in brain, lung, placenta, smooth muscle cells, and malignant tumor tissues including oral melanoma and renal carcinoma (1). MMP-16 has been shown to activate proMMP-2 and degrade various ECM components including native collagens (2, 3). MMP-16 has been proposed to possess the potential to directly enhance the growth and invasiveness of cells in vivo, two critical processes for development and carcinogenesis (4). Structurally, MMP-16 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail (1). The structure of the catalytic domain in complex with a hydroxamate inhibitor has been solved (5). The rhMMP-16PC consists of the pro and catalytic domains, which can be activated by treatment with furin.

References

  1. Takino, T. et al. (1995) J. Biol. Chem. 270:23013.
  2. Shofuda, K. et al. (1997) J. Biol. Chem. 272:9749.
  3. Shimada, T. et al. (1999) Eur. J. Biochem. 262:907.
  4. Kang, T. et al. (2000) FASEB J. 14:2559.
  5. Lang, R. et al. (2004) J. Mol. Biol. 336:213.

Long Name

Matrix Metalloproteinase 16/Membrane Type 3 MMP

Alternate Names

MMP16, MT3-MMP

Entrez Gene IDs

4325 (Human)

Gene Symbol

MMP16

UniProt

P51512

Additional MMP-16/MT3-MMP Products

Product Documents for Human MMP-16/MT3-MMP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP-16/MT3-MMP Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human MMP-16/MT3-MMP Antibody

Customer Reviews for Human MMP-16/MT3-MMP Antibody (1)

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  • Human MMP-16/MT3-MMP Antibody
    Name: Ruhul Choudhury
    Application: immunofluorescence - paraffin
    Sample Tested: First trimester decidua
    Species: Human
    Verified Customer | Posted 03/18/2017
    40X magnification. MMP16 is expressed by decidual stromal cells.
    Sodium citrate antigen retrieval. Permeabilized with 0.5% triton X. Primary antibody concentration - 10 ug/ml Secondary antibody - Rabbit Anti-Goat Alexa Fluor® 568 at 4 ug/ml.
    Human MMP-16/MT3-MMP Antibody AF1785

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