Human/Mouse HAND2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF3876N Datasheet / COA / SDS

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AF3876N-100UG

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Human/Mouse HAND2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse HAND2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human HAND1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HAND2
Met1-Gln217
Accession # P61296

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HAND2

HAND2 (also known as dHAND, Thing-2 and Hed) is a 24 kDa, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.

Long Name

Heart And Neural Crest Derivatives Expressed 2

Entrez Gene IDs

9464 (Human); 15111 (Mouse); 64637 (Rat)

Alternate Names

BHLHA26; bHLHa26MGC125303; dHand; DHAND2; Ehand2; FLJ16260; HAND2; heart and neural crest derivatives expressed 2; heart, autonomic nervous system and neural crestderivatives-expressed protein 2; Hed; MGC125304; Th2; Thing2