Human/Mouse KLF5 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF3758X Datasheet / COA / SDS

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AF3758X-100UG

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All KLF5 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse KLF5 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse KLF5 in Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) KLF4, rhKLF6, recombinant mouse (rm) KLF4 and rmKLF15 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human KLF5
Leu85-Ala177
Accession # Q13887

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: KLF5

Human KLF5, also known as basic transcription element binding protein 2 (BTEB2), is a widely expressed 51 kDa (457 aa) member of the kruppel C2H2-type zinc finger protein family. KLF5 contains three C2H2-type zinc fingers at the carboxyl-terminus that preferentially bind to cis-DNA elements that are GC rich. It is a transcription activator that promotes cell proliferation and oncogenesis. Over the region used as immunogen, human, mouse and canine KLF5 share 95% aa sequence identity.

Long Name

Kruppel-Like Factor 5

Entrez Gene IDs

688 (Human)

Alternate Names

Basic transcription element-binding protein 2; BTEB2; BTE-binding protein 2; CKLFGC-box-binding protein 2; Colon krueppel-like factor; colon kruppel-like factor; GC box binding protein 2; IKLFbasic transcription element binding protein 2; Intestinal-enriched krueppel-like factor; intestinal-enriched kruppel-like factor; KLF5; Krueppel-like factor 5; Kruppel-like factor 5 (intestinal); Transcription factor BTEB2