Meprin beta Subunit/MEP1B Antibody (289731)

Detection of Human and Mouse Meprin beta Subunit/ MEP1B by Western Blot.

Western blot shows lysates of human intestine tissue and mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Meprin beta Subunit/MEP1B at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Meprin beta Subunit/MEP1B in Human Intestine.

Meprin beta Subunit/MEP1B was detected in immersion fixed paraffin-embedded sections of human intestine using Human/Mouse Meprin beta Subunit/MEP1B Monoclonal Antibody (Catalog # MAB28951) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hemotoxylin (blue). Specific staining was localized to the Brush border of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in mouse samples

Detection of Meprin beta Subunit/MEP1B by Western Blot

Representative proteins in ULF-EVs whose abundance increased with pregnancy development also significantly increased after ULF-EVs treatment of pTr2 cells. A Western blot analysis of MUC4, ACP5 and MEP1B in ULF-EVs. B Relative abundance of MUC4, ACP5 and MEP1B in pTr2 cells treated with ULF-EVs. C Images of the embryo-maternal interface stained with MEP1B antibodies. MEP1B was obviously expressed in the endometrium and trophoblast. The scale bar indicates 100 nm. D Quantitative analysis of MEP1B by assessing the average integrated optical density (IOD) in the endometrium. The data are displayed as mean ± SD and different lowercase letters correspond to significant differences at the p < 0.05 threshold. E Quantitative analysis of MEP1B by assessing IOD in the trophoblast. Asterisks indicate significant differences (mean ± SD) between 12 and 15P (**p < 0.01). F The relative expression level of MEP1B mRNA was determined by qRT-PCR. The data are displayed as mean ± SD and asterisk indicate significant differences (*p < 0.05) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36882792), licensed under a CC-BY license. Not internally tested by R&D Systems.