Human/Mouse/Rat Beclin 1 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB5295U Datasheet / COA / SDS

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FAB5295U-100UG

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Human/Mouse/Rat Beclin 1 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human Beclin 1 in direct ELISAs. Detects human, mouse, and rat Beclin 1 in Western blots. In direct ELISAs,
no cross-reactivity with recombinant human ATG3, 4A, 4B, 5, 7, 10, 12, or 16L1 is observed.

Source

Monoclonal Mouse IgG2b Clone # 669922

Purification

Protein A or G purified

Immunogen

E.coli-derived recombinant human Beclin 1
Met12-Gln239
Accession # Q14457

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Beclin 1

Beclin 1 (Bcl-2 interacting coiled-coil protein; also know as ATG6) is a 60 kDa member of the Beclin family of proteins. It is part of a lipid-kinase complex that includes UVRAG and PI3 and induces autophagy; in the presence of Bcl-2, autophagy is repressed. Beclin 1 is widely expressed but abundantly found in mammary epithelium, glia and neurons. Human Beclin 1 is 450 amino acids (aa) in length and contains a Bcl-2 binding domain (aa 88‑150), a nuclear export signal motif (aa 180‑189), a coiled-coil region (aa 142‑270), and an evolutionary conserved domain (aa 244‑337). There is one potential alternate start site at Met88. Over aa 12‑239, human Beclin 1 is 98% aa identical to mouse Beclin 1.

Long Name

Beclin1, Autophagy-related

Entrez Gene IDs

8678 (Human); 56208 (Mouse); 114558 (Rat)

Alternate Names

ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30