Human/Mouse/Rat BTF3 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF5747G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5747G-100UG

Save 15% on Select RUO Reagents. See Details.

All BTF3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat BTF3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat BTF3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BTF3
Lys46-Asn206
Accession # P20290

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BTF3

BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex beta /NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ER alpha ) stimulation of gene activation by binding to both ligand activated and non-ligand activated ER alpha. Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108.

Long Name

Basic Transcription Factor 3

Entrez Gene IDs

689 (Human); 218490 (Mouse); 498293 (Rat)

Alternate Names

basic transcription factor 3; BETA-NAC; BTF3; BTF3a; BTF3b; NACB; nascent-polypeptide-associated complex beta polypeptide; RNA polymerase B transcription factor 3; transcription factor BTF3