Human/Mouse/Rat BTF3 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5747T Datasheet / COA / SDS

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AF5747T-100UG

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Human/Mouse/Rat BTF3 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat BTF3 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BTF3
Lys46-Asn206
Accession # P20290

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BTF3

BTF3 (basic transcription factor 3; also nascent polypeptide-associated complex beta /NACB) is a member of the NAC-beta family of transcription factors. It is ubiquitously expressed and involved in the initiation of gene transcription by RNA polymerase. BTF3 also appears to impact estrogen receptor alpha (ER alpha ) stimulation of gene activation by binding to both ligand activated and non-ligand activated ER alpha. Human BTF3 is 206 amino acids (aa) in length and contains one potential phosphorylation site at Ser30, and a NACB domain (aa 82-147). There are multiple potential splice variants. Two variants show alternate start sites, one at Met45 (called BTF3b), and another at Met108.

Long Name

Basic Transcription Factor 3

Entrez Gene IDs

689 (Human); 218490 (Mouse); 498293 (Rat)

Alternate Names

basic transcription factor 3; BETA-NAC; BTF3; BTF3a; BTF3b; NACB; nascent-polypeptide-associated complex beta polypeptide; RNA polymerase B transcription factor 3; transcription factor BTF3