DDX1 Antibody (2220A)

R&D Systems | Catalog # MAB9615

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2220A
View all DDX1 Primary Antibodies »

Product Specifications

Immunogen

Human/mouse/rat DDX1 synthetic Peptide
Accession # Q92499

Specificity

Detects human, mouse, and rat DDX1 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DDX1 Antibody (2220A)

Detection of Human, Mouse, and Rat DDX1 antibody by Western Blot.

Detection of Human, Mouse, and Rat DDX1 by Western Blot.

Western blot shows lysates of Y-79 human retinoblastoma cell line, A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for DDX1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DDX1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

DDX1 in HeLa Human Cell Line.

DDX1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
DDX1 antibody in Human Breast Cancer Tissue by Immunohistochemistry (IHC-P).

DDX1 in Human Breast Cancer Tissue.

DDX1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Western Blot Shows DDX1 Specificity Using Knockout Cell Line.

Western Blot Shows DDX1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma parental cell line and DDX1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) followed by HRP-conjugated secondary antibody. A specific band was detected for DDX1 at approximately 82.4 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. Primary antibody dilution used: 0.5 µg/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.
Detection of DDX1 by Immunoprecipitation.

Detection of DDX1 by Immunoprecipitation.

U2OS human osteosarcoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) pre-coupled to Dynabeads Protein A. Immunoprecipitated DDX1 was detected in Western Blot with a mouse DDX1 antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

Applications for DDX1 Antibody (2220A)

Application
Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Immunoprecipitation

2 µg/1 mg cell lysate
Sample: Cell lysate of U2OS cell line

Knockout Validated

DDX1 is specifically detected in U2OS parental cell line but is not detectable in knockout U2OS cell line

Western Blot

0.5 µg/mL
Sample: Y‑79 human retinoblastoma cell line, A431 human epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, Neuro‑2A mouse neuroblastoma cell line, and Rat‑2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DDX1

DEAD (Asp-Glu-Ala-Asp) box helicase 1 (DDX1) is a putative RNA helicase gene.  RNA helicases are involved in various cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. It has been proposed that DDX1 plays a role in RNA clearance at DNA double-strand breaks. It has also been reported as a coactivator of the NF-kappa-B promoter. DDX1 also acts as a positive transcriptional regulator of cyclin CCND2 expression. Human DDX1 has 97% homology to mouse and rat DDX1. Expression in embryos and testis suggest that DDX1 mediates cell growth and development. DDX1 has been proposed as a diagnostic marker for early recurrence in primary breast cancer.

Long Name

DEAD-Box Helicase 1

Alternate Names

DBP-RB, DEAD Box-1, DEAD/H-Box Helicase 1, UKVH5d

Entrez Gene IDs

1653 (Human); 104721 (Mouse); 84474 (Rat)

Gene Symbol

DDX1

UniProt

Q92499

Additional DDX1 Products

Product Documents for DDX1 Antibody (2220A)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for DDX1 Antibody (2220A)

For research use only

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Protocols

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