Human/Mouse/Rat DDX1 Antibody

Detection of Human, Mouse, and Rat DDX1 by Western Blot. Western blot shows lysates of Y-79 human retinoblastoma cell line, A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for DDX1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DDX1 in HeLa Human Cell Line. DDX1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

DDX1 in Human Breast Cancer Tissue. DDX1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Shows DDX1 Specificity Using Knockout Cell Line. Western blot shows lysates of U2OS human osteosarcoma parental cell line and DDX1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) followed by HRP-conjugated secondary antibody. A specific band was detected for DDX1 at approximately 82.4 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. Primary antibody dilution used: 0.5 µg/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of DDX1 by Immunoprecipitation. U2OS human osteosarcoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Rabbit Anti-Human/Mouse/Rat DDX1 Monoclonal Antibody (Catalog # MAB9615) pre-coupled to Dynabeads Protein A. Immunoprecipitated DDX1 was detected in Western Blot with a mouse DDX1 antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).