Human/Mouse/Rat GBL Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4004R Datasheet / COA / SDS

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AF4004R-100UG

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Human/Mouse/Rat GBL Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat G beta L in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GÎ²L
Met1-Gly327
Accession # Q9BVC4

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GBL

G-protein beta -subunit-like (G beta L), also known as mLST8, is a subunit of the Target of Rapamycin (TOR) complex. G beta L contains 7 WD-40 repeats, and binds to the kinase domain of TOR in both the Raptor (Rapamycin-sensitive)-and Rictor (Rapamycin-insensitive)-containing complexes. G beta L is necessary for the full activation of the TOR kinase, and phosphorylation of many downstream proteins, including 4E-BP1 and p70^S6 kinase.

Long Name

G protein beta subunit-like

Entrez Gene IDs

64223 (Human)

Alternate Names

G protein beta subunit-like; gable; GbetaL; GBL; GBLPop3; Lst8; LST8MGC111011; Mammalian lethal with SEC13 protein 8; mLST8; MTOR associated protein, LST8 homolog (S. cerevisiae); POP3; Protein GbetaL; target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1