Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2018B
View all HHEX Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HHEX
Thr111-Gly270
Accession # Q03014

Specificity

Detects human HHEX in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat HHEX Antibody

Detection of Human, Mouse, and Rat HHEX antibody by Western Blot.

Detection of Human, Mouse, and Rat HHEX by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, BaF3 mouse pro-B cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat HHEX Monoclonal Antibody (Catalog # MAB83771) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HHEX at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HHEX antibody in K562 Human Cell Line by Immunocytochemistry (ICC).

HHEX in K562 Human Cell Line.

HHEX was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Rabbit Anti-Human/Mouse/Rat HHEX Polyclonal Antibody (Catalog # MAB83771) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of HHEX antibody in A549 Human Cell Line antibody by Flow Cytometry.

Detection of HHEX in A549 Human Cell Line by Flow Cytometry.

A549 human lung carcinoma cell line was stained with Rabbit Anti-Human/Mouse/Rat HHEX Monoclonal Antibody (Catalog # MAB83771, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

HHEX antibody in iBJ6 iPS Cell Line by Immunocytochemistry (ICC).

HHEX in iBJ6 iPS Cell Line.

HHEX was detected in immersion fixed iBJ6 iPS cell line differentiated into hepatocytes using Rabbit Anti-Human/Mouse/Rat HHEX Polyclonal Antibody (Catalog # MAB83771) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse/Rat HHEX Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: A549 human lung carcinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Immunocytochemistry

2-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line and immersion fixed iBJ6 iPS cell line differentiated into hepatocytes

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, BaF3 mouse pro-B cell line, and H4‑II‑E‑C3 rat hepatoma cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB83771 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HHEX

Hematopoietically-expressed homeobox protein (HHEX), also known as HEX, PRH and PRHX, is a 35-40 kDa member of the Homeobox family of transcription factors.  Family members are distinguished by an evolutionarily conserved DNA-binding homeodomain of 60 amino acids (aa), which for HHEX spans aa 137-196.  Human HHEX was initially isolated from hematopoietic tissue, and is present in several hematopoietic progenitors, where its expression is down-regulated during terminal cell differentiation.  HHEX is also expressed in the anterior visceral endoderm during early mouse development, and in some adult tissues of endodermal origin, including liver, lung and thyroid.  HHEX knockout in mice is embryonic lethal, with impaired forebrain, liver and thyroid development.  Human HHEX is 270 aa in length, and over aa 111-270, shares 93% and 95% identity with mouse and rat HHEX, respectively.

Long Name

Hematopoietically Expressed Homeobox

Alternate Names

Hex, HMPH, HOX11L-PEN, PRH, PRHX

Entrez Gene IDs

3087 (Human); 15242 (Mouse); 79237 (Rat)

Gene Symbol

HHEX

UniProt

Q03014

Additional HHEX Products

Product Documents for Human/Mouse/Rat HHEX Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat HHEX Antibody

For research use only

Citations for Human/Mouse/Rat HHEX Antibody

Customer Reviews for Human/Mouse/Rat HHEX Antibody (2)

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2 Customer Ratings
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  • Human/Mouse/Rat HHEX Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HT-29 human colon adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 07/09/2022
    Human/Mouse/Rat HHEX Antibody MAB83771
  • Human/Mouse/Rat HHEX Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Osteoblasts
    Species: Mouse
    Verified Customer | Posted 11/03/2021
    Human/Mouse/Rat HHEX Antibody MAB83771

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