JNK2 Antibody (252320)

R&D Systems | Catalog # MAB1846

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry

Cited:

Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 252320
View all JNK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human JNK2 isoform 2

Specificity

Detects human, mouse, and rat JNK2, expressed as p46 JNK (isoforms 2 and/or 3, both 382 aa) and p54 JNK (isoforms 1 and/or 4, both 424 aa) in Western blots. Does not detect recombinant JNK1 or JNK3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for JNK2 Antibody (252320)

Detection of Human, Mouse, and Rat JNK2 antibody by Western Blot.

Detection of Human, Mouse, and Rat JNK2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK2 were detected at approximately 46 and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
JNK2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

JNK2 in HeLa Human Cell Line.

JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western Blot Shows Human JNK2 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human JNK2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and JNK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for JNK2 at approximately 45 and 54 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

JNK2 in C2C12 Mouse Cell Line.

JNK2 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat JNK2 Monoclonal Antibody (Catalog # MAB1846) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for JNK2 Antibody (252320)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and C2C12 mouse myoblast cell line

Knockout Validated

JNK2 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in JNK2 knockout HEK293T cell line.

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line

Reviewed Applications

Read 2 reviews rated 5 using MAB1846 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: JNK2

The c-Jun N-terminal kinases (JNKs) are encoded by three genes: JNK1, JNK2, and JNK3. Members of the MAPK superfamily, JNKs are activated by environmental stresses and inflammatory cytokines. JNK1, also known as SAPK1 gamma and MAPK8, is expressed as four isoforms generated by alternative splicing. JNK1 is activated by dual phosphorylation at T183 and Y185 by the MAPK kinases MKK4 and/or MKK7.

Long Name

C-Jun N-terminal Kinase 2

Alternate Names

MAPK9, SAPK1 alpha

Entrez Gene IDs

5601 (Human); 243911 (Mouse); 50658 (Rat)

Gene Symbol

MAPK9

Additional JNK2 Products

Product Documents for JNK2 Antibody (252320)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for JNK2 Antibody (252320)

For research use only

Citations for JNK2 Antibody (252320)

Customer Reviews for JNK2 Antibody (252320) (2)

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  • Human/Mouse/Rat JNK2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Retina
    Species: Rat and Mouse
    Verified Customer | Posted 12/31/2016
    Retina samples from rat (1), wild-type mouse (2), and JNK2 ko mouse (3) probed for JNK2. Major band at 54 kDa and additional band at 46 kDa are as expected from prior publications. Absence of bands in lanes with JNK2 ko sample demonstrates specificity.
    JNK2 Ab was used at 1:2000 over night, followed by peroxidase conjugated donkey anti mouse secondary antibody and chemiluminescence detection.
    JNK2 Antibody (252320) MAB1846
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cancer cell whole cell lysate
    Species: Human
    Verified Customer | Posted 10/04/2015
    JNK2 expression in MDA-MB-231 in response to carboplatin treatment.
    JNK2 Antibody (252320) MAB1846

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Protocols

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