Human/Mouse/Rat MDM2/HDM2 Antibody

Catalog # Availability Size / Price Qty
MDM2/HDM2 in Human Kidney.
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Product Details
Citations (9)
Supplemental Products

Human/Mouse/Rat MDM2/HDM2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat MDM2.
Polyclonal Rabbit IgG
Antigen Affinity-purified
E. coli-derived recombinant human MDM2
Accession # Q00987
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

MDM2/HDM2 in Human Kidney. MDM2/HDM2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse/Rat MDM2/HDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei for epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry MDM2 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

MDM2 in Mouse Kidney. MDM2 was detected in perfusion fixed frozen sections of mouse kidney using Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Human MDM2 antibody by Western Blot. View Larger

Detection of Human MDM2 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, and CEM human T-lymphoblastoid cell line untreated (-) or treated (+) with 5 µM LLnV for 1 hour. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDM2/HDM2

MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally up‑regulates the expression of its own inhibitor, MDM2.

Long Name
Double Minute 2 Protein
Entrez Gene IDs
4193 (Human); 17246 (Mouse)
Alternate Names
ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2

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Citations for Human/Mouse/Rat MDM2/HDM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Cardiac GRK2 Protein Levels Show Sexual Dimorphism during Aging and Are Regulated by Ovarian Hormones
    Authors: AC Arcones, MR Martínez-C, R Vila-Bedma, C Yáñez, I Lladó, AM Proenza, F Mayor, C Murga
    Cells, 2021-03-17;10(3):.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation
    Authors: A Colmenero-, MA Gómez-Muño, I Rodríguez-, A Amador-Álv, KO Henrich, D Pascual-Va, K Okonechnik, E Rivas, F Westermann, R Pardal, FM Vega
    Cancers (Basel), 2020-11-20;12(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Adverse Effects on ?-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function
    Authors: R Schreckenb, P Bencsik, M Weber, Y Abdallah, C Csonka, K Gömöri, K Kiss, J Pálóczi, J Pipis, M Sárközy, P Ferdinandy, R Schulz, KD Schlüter
    J Am Heart Assoc, 2017-12-22;6(12):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
    Authors: Phesse, T J, Myant, K B, Cole, A M, Ridgway, R A, Pearson, H, Muncan, V, van den Brink, G R, Vousden, K H, Sears, R, Vassilev, L T, Clarke, A R, Sansom, O J
    Cell Death Differ, 2014-02-28;21(6):956-66.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
    J Hematol Oncol, 2012-09-18;5(0):57.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells.
    Authors: Surget S, Chiron D, Gomez-Bougie P, Descamps G, Menoret E, Bataille R, Moreau P, Le Gouill S, Amiot M, Pellat-Deceunynck C
    Cancer Res, 2012-06-27;72(17):4562-73.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development.
    Authors: Post SM, Quintas-Cardama A, Pant V, Iwakuma T, Hamir A, Jackson JG, Maccio DR, Bond GL, Johnson DG, Levine AJ, Lozano G
    Cancer Cell, 2010-09-14;18(3):220-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
    Authors: Rozan LM, McDonald ER, Navaraj A, Matthew EM, Dicker DT, El-Deiry WS
    J. Biol. Chem., 2006-11-22;282(5):3273-81.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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