Human/Mouse/Rat MDM2/HDM2 Antibody

Catalog #: AF1244
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1244
AF1244-SP

Request a Quote Bulk Orders

3 Images

Product Details

Citations (9)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat MDM2/HDM2 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MDM2.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MDM2
Asn3-Pro491
Accession # Q00987

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

MDM2/HDM2 in Human Kidney. MDM2/HDM2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human/Mouse/Rat MDM2/HDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei for epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry

View Larger

MDM2 in Mouse Kidney. MDM2 was detected in perfusion fixed frozen sections of mouse kidney using Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot

View Larger

Detection of Human MDM2 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line, MCF-7 human breast cancer cell line, and CEM human T-lymphoblastoid cell line untreated (-) or treated (+) with 5 µM LLnV for 1 hour. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MDM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1244), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MDM2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDM2/HDM2

MDM2 is a key regulator of p53 tumor suppressor protein activity and stability. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 stability by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally up‑regulates the expression of its own inhibitor, MDM2.

Long Name

Double Minute 2 Protein

Entrez Gene IDs

4193 (Human); 17246 (Mouse)

Alternate Names

ACTFS; HDM2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat MDM2/HDM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

Cardiac GRK2 Protein Levels Show Sexual Dimorphism during Aging and Are Regulated by Ovarian Hormones
Authors: AC Arcones, MR Martínez-C, R Vila-Bedma, C Yáñez, I Lladó, AM Proenza, F Mayor, C Murga
Cells, 2021-03-17;10(3):.
Species: Mouse, Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Identification of VRK1 as a New Neuroblastoma Tumor Progression Marker Regulating Cell Proliferation
Authors: A Colmenero-, MA Gómez-Muño, I Rodríguez-, A Amador-Álv, KO Henrich, D Pascual-Va, K Okonechnik, E Rivas, F Westermann, R Pardal, FM Vega
Cancers (Basel), 2020-11-20;12(11):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Adverse Effects on ?-Adrenergic Receptor Coupling: Ischemic Postconditioning Failed to Preserve Long-Term Cardiac Function
Authors: R Schreckenb, P Bencsik, M Weber, Y Abdallah, C Csonka, K Gömöri, K Kiss, J Pálóczi, J Pipis, M Sárközy, P Ferdinandy, R Schulz, KD Schlüter
J Am Heart Assoc, 2017-12-22;6(12):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Endogenous c-Myc is essential for p53-induced apoptosis in response to DNA damage in vivo.
Authors: Phesse, T J, Myant, K B, Cole, A M, Ridgway, R A, Pearson, H, Muncan, V, van den Brink, G R, Vousden, K H, Sears, R, Vassilev, L T, Clarke, A R, Sansom, O J
Cell Death Differ, 2014-02-28;21(6):956-66.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 (cis-imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas.
J Hematol Oncol, 2012-09-18;5(0):57.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Cell death via DR5, but not DR4, is regulated by p53 in myeloma cells.
Authors: Surget S, Chiron D, Gomez-Bougie P, Descamps G, Menoret E, Bataille R, Moreau P, Le Gouill S, Amiot M, Pellat-Deceunynck C
Cancer Res, 2012-06-27;72(17):4562-73.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A high-frequency regulatory polymorphism in the p53 pathway accelerates tumor development.
Authors: Post SM, Quintas-Cardama A, Pant V, Iwakuma T, Hamir A, Jackson JG, Maccio DR, Bond GL, Johnson DG, Levine AJ, Lozano G
Cancer Cell, 2010-09-14;18(3):220-30.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
CARPs are ubiquitin ligases that promote MDM2-independent p53 and phospho-p53ser20 degradation.
Authors: Rozan LM, McDonald ER, Navaraj A, Matthew EM, Dicker DT, El-Deiry WS
J. Biol. Chem., 2006-11-22;282(5):3273-81.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot