Human/Mouse/Rat Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Alexa Fluor® 350-conjugated Antibody

Catalog #: AF3918U Datasheet / COA / SDS

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AF3918U-100UG

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Human/Mouse/Rat Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat ribosomal protein S6 when dually phosphorylated at S235 and S236.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human, mouse and rat Ribosomal Protein S6 S235/S236 sites

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ribosomal Protein S6/RPS6

40S ribosomal protein S6 is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. S6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens.

Entrez Gene IDs

6194 (Human); 20104 (Mouse); 29304 (Rat)

Alternate Names

Phosphoprotein NP33, 40S ribosomal protein S6; pS6; Ribosomal Protein S6; RPS6; S6