XIAP (X-chromosome linked inhibitor of apoptosis) is a member of the apoptosis (IAP) family of proteins that inhibit caspases. The BIR2 domain of XIAP inhibits caspase-3 and caspase-7. The ability of XIAP to inhibit caspases is prevented by SMAC/Diablo through binding to XIAP-BIR2 and -BIR3 domains.
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human XIAP
Met1-Ser497 (Ser162Cys)
Accession # P98170
Met1-Ser497 (Ser162Cys)
Accession # P98170
Specificity
Detects human and mouse XIAP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for XIAP Antibody [Biotin]
Detection of Human and Mouse XIAP by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human XIAP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF8221) followed by Streptavidin-HRP (Catalog # DY998). A specific band was detected for XIAP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for XIAP Antibody [Biotin]
Application
Recommended Usage
Western Blot
0.25 µg/mL
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: XIAP
Long Name
X-linked Inhibitor of Apoptosis
Alternate Names
BIRC4
Gene Symbol
XIAP
UniProt
Additional XIAP Products
Product Documents for XIAP Antibody [Biotin]
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for XIAP Antibody [Biotin]
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
