Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 940906
View all MYH7 Primary Antibodies »

Product Specifications

Immunogen

Human MYH7 synthetic peptide
Accession # P12883

Specificity

Detects human MYH7 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human MYH7 Antibody

Detection of Human MYH7 antibody by Western Blot.

Detection of Human MYH7 by Western Blot.

Western blot shows lysates of human skeletal muscle tissue and human heart (atrium) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MYH7 antibody in Human Heart by Immunohistochemistry (IHC-P).

MYH7 in Human Heart.

MYH7 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MYH7 Antibody

Application
Recommended Usage

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart

Western Blot

0.5 µg/mL
Sample: Human skeletal muscle tissue and human heart (atrium) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYH7

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha  and  beta  display 93% homology but have significantly different enzymatic properties, with  alpha  having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta. Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Long Name

Myosin Heavy Chain 7, Cardiac Muscle, Beta

Alternate Names

CMD1S, CMH1, MPD1, MYHCB, Myosin-7, SPMD, SPMM

Entrez Gene IDs

4625 (Human); 140781 (Mouse); 29557 (Rat)

Gene Symbol

MYH7

UniProt

P12883

Additional MYH7 Products

Product Documents for Human MYH7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MYH7 Antibody

For research use only

Related Research Areas

Cardiac Stem Cell Markers

Citations for Human MYH7 Antibody

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Protocols

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