Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 671037
View all Myogenin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173

Specificity

Detects human Myogenin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Myogenin Antibody

Detection of Human Myogenin antibody by Western Blot.

Detection of Human Myogenin by Western Blot.

Western blot shows lysates of Rh30 human rhabdomyosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myogenin at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Myogenin antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

Myogenin in C2C12 Mouse Cell Line.

Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cell line using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB66861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Myogenin Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed differentiated C2C12 mouse myoblast cell line

Western Blot

1 µg/mL
Sample: Rh30 human rhabdomyosarcoma cell line

Reviewed Applications

Read 1 review rated 4 using MAB66861 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Myogenin

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Alternate Names

BHLHC3, MYF4, MYOG

Entrez Gene IDs

4656 (Human); 17928 (Mouse); 29148 (Rat)

Gene Symbol

MYOG

UniProt

P15173

Additional Myogenin Products

Product Documents for Human Myogenin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Myogenin Antibody

For research use only

Citations for Human Myogenin Antibody

Customer Reviews for Human Myogenin Antibody (1)

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  • Name: Zachary Patinkin
    Application: Simple Western
    Sample Tested: Human Skeletal Muscle Lysate; 3T3-L1 cell lysate
    Species: Human
    Verified Customer | Posted 02/25/2015
    Positive Control: Human Skeletal Muscle Tissue; negative control: 3T3-L1 Cell Lysate. Specific band at 47 kDa.
    Human Myogenin Antibody MAB66861

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Protocols

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FAQs

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