Human Neuroligin 3/NLGN3 Antibody (566209)

R&D Systems | Catalog # MAB6088

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 566209
View all Neuroligin 3/NLGN3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Neuroligin 3
Gln38-Leu709
Accession # NP_061850

Specificity

Detects human Neuroligin 3 in direct ELISAs.  In this format, 100% cross-reactivity with recombinant human  (rh) Neuroligin 3 variant 2 and recombinant rat Neuroligin 3 variant 2 and no cross-reactivity with rhNeuroligin 1 variant 2, rhNeuroligin 2, rhNeuroligin 4, recombinant rat Neuroligin 1, 1 variant 2, 2 variant 2, or 3 variant 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Neuroligin 3/NLGN3 Antibody (566209)

Neuroligin 3/NLGN3 antibody in Human Brain by Immunohistochemistry (IHC-P).

Neuroligin 3/NLGN3 in Human Brain.

Neuroligin 3/NLGN3 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Human Neuroligin 3/NLGN3 Monoclonal Antibody (Catalog # MAB6088) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Neuroligin 3/NLGN3 Antibody (566209)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed 7-day differentiated rat cortical stem cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Reviewed Applications

Read 1 review rated 5 using MAB6088 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neuroligin 3/NLGN3

NLGN3 (Neuroligin 3; also gliotactin homolog) is a 110-114 kDa member of the type-B carboxyesterase/lipase family of proteins.  It is a neuronal transmembrane protein that forms Ca++-dependent intercellular junctions with short beta -neurexin isoforms.  This seems to contribute to both glutamatergic and GABAergic synapse formation.  Mutations in NLGN3 are associated with a reduction in protein expression and the occurrence of autism.  Mature human NLGN3 is an 811 amino acid (aa) type I transmembrane protein.  It contains a 672 aa extracellular domain (ECD) (aa 38-709), plus a 118 aa cytoplasmic region.  The ECD possesses a nonfunctional carboxyesterase domain (aa 41-625).  Multiple splice variants exist.  There is a deletion of aa 153-172 that may also be accompanied by an alternative start site at Met118, and a deletion of aa 153-192 that may also be accompanied by a five Lys substitution for aa 554-848.  Over aa 38-709, human NLGN3 shares 99% aa identity with mouse NLGN3.        

Alternate Names

ASPGX1, AUTSX1, HNL3, NL3, NLGN3

Entrez Gene IDs

54413 (Human)

Gene Symbol

NLGN3

UniProt

NP_061850

Additional Neuroligin 3/NLGN3 Products

Product Documents for Human Neuroligin 3/NLGN3 Antibody (566209)

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Product Specific Notices for Human Neuroligin 3/NLGN3 Antibody (566209)

For research use only

Citations for Human Neuroligin 3/NLGN3 Antibody (566209)

Customer Reviews for Human Neuroligin 3/NLGN3 Antibody (566209) (1)

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  • Human Neuroligin 3/NLGN3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 05/22/2022
    Human Neuroligin 3/NLGN3 Antibody (566209) MAB6088

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